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Differentiation of Mycobacterium species by analysis of the heat-shock protein 65 gene (hsp65)

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Authors
Kim, Hong; Kim, Sun-Hyun; Shim, Tae-Sun; Kim, Mi-na; Bai, Gill-Han; Park, Young-Gil; Lee, Sueng-Hyun; Chae, Gue-Tae; Cha, Chang-Yong; Kook, Yoon-Hoh; Kim, Bum-Joon
Issue Date
2005-07-15
Publisher
Society for General Microbiology
Citation
Int J Syst Evol Microbiol. 55(Pt 4), 1649-1656
Keywords
Bacterial Proteins/*genetics*Bacterial Typing TechniquesChaperonins/*geneticsDNA, Bacterial/analysisDNA, Ribosomal/analysisDeoxyribonucleases, Type II Site-Specific/metabolismHumansMolecular Sequence DataMycobacterium/*classification/geneticsMycobacterium tuberculosis/classification/geneticsPhylogenyPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthRNA, Ribosomal, 16S/geneticsSequence Analysis, DNASpecies Specificity
Abstract
The nucleotide sequences (604 bp) of partial heat-shock protein genes (hsp65) from 161 Mycobacterium strains containing 56 reference Mycobacterium species and 105 clinical isolates were determined and compared. hsp65 sequence analysis showed a higher degree of divergence between Mycobacterium species than did 16S rRNA gene analysis. Generally, the topology of the phylogenetic tree based on the hsp65 DNA sequences was similar to that of the 16S rRNA gene, thus revealing natural relationships among Mycobacterium species. When a direct sequencing protocol targeting 422 bp sequences was applied to 70 non-tuberculous mycobacterium (NTM) clinical isolates, all NTMs were clearly identified. In addition, an XhoI PCR restriction fragment length polymorphism analysis method for the differentiation of Mycobacterium tuberculosis complex from NTM strains was developed during this study. The results obtained suggest that 604 bp hsp65 sequences are useful for the phylogenetic analysis and species identification of mycobacteria.
ISSN
1466-5026 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16014496

http://hdl.handle.net/10371/67895
DOI
https://doi.org/10.1099/ijs.0.63553-0
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College of Medicine/School of Medicine (의과대학/대학원)Microbiology (미생물학전공)Journal Papers (저널논문_미생물학전공)
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