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Ischemia-induced ribosomal protein S3 expressional changes and the neuroprotective effect against experimental cerebral ischemic damage

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dc.contributor.authorHwang, In Koo-
dc.contributor.authorYoo, Ki-Yeon-
dc.contributor.authorKim, Dae Won-
dc.contributor.authorKim, So Young-
dc.contributor.authorPark, Jun Hong-
dc.contributor.authorRyoo, Zae Young-
dc.contributor.authorKim, Joon-
dc.contributor.authorChoi, Soo Young-
dc.contributor.authorWon, Moo-Ho-
dc.date.accessioned2010-07-01T07:14:39Z-
dc.date.available2010-07-01T07:14:39Z-
dc.date.issued2008-01-25-
dc.identifier.citationJ Neurosci Res. 86(8):1823-1835en
dc.identifier.issn1097-4547 (Electronic)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18214992-
dc.identifier.urihttp://hdl.handle.net/10371/68168-
dc.description.abstractSome ribosomal proteins are important regulators of development and DNA repair. However, few studies have been conducted on ribosomal protein S3 (rpS3) in the ischemic hippocampus. In the present study, we investigated ischemia-induced changes in rpS3 immunoreactivity, rpS3 mRNA, and protein levels in the hippocampal CA1 region of Mongolian gerbil after 5 min of transient forebrain ischemia. RpS3 immunoreactivity and its protein level were found to be significantly elevated at 6 hr after ischemia/reperfusion and then continuously decreased with time. RT-PCR analysis also showed that rpS3 mRNA levels were significantly elevated in CA1 at 6 hr after transient ischemia. In addition, during the course of this study, we developed a delivery vector (Pep-1) and its rpS3 fusion protein (Pep-1-rpS3) to elucidate the role of rpS3 in ischemia-induced damage. Pep-1-rpS3 administration to ischemic animals significantly and dose dependently increased neuronal survival in the stratum pyramidale of CA1. Moreover, Pep-1-rpS3 treatment reduced terminal deoxynucleotidyl dUTP nick-end labeling-positive CA1 pyramidal cell numbers in the stratum pyramidale. To elucidate how Pep-1-rpS3 ameliorates ischemic damage, changes in 4-hydroxy-2-nonnenal (HNE; an indicator of lipid peroxidation) immunoreactivity and protein levels were investigated. HNE levels and immunoreactivities in Pep-1-rpS3-treated ischemic animals were lower than in corresponding Pep-1-treated ischemic animals. These results indicate that rpS3 has a neuroprotective effect in the brain exposed to ischemia.en
dc.language.isoenen
dc.publisherWiley-Blackwellen
dc.subjectAnimalsen
dc.subjectBase Sequenceen
dc.subjectBrain Ischemia/genetics/*metabolism/*prevention & controlen
dc.subjectGene Expression Regulation/*physiologyen
dc.subjectGerbillinaeen
dc.subjectHumansen
dc.subjectMaleen
dc.subjectMiceen
dc.subjectMice, Inbred BALB Cen
dc.subjectMolecular Sequence Dataen
dc.subjectNeuroprotective Agents/*metabolismen
dc.subjectRibosomal Proteins/*biosynthesis/geneticsen
dc.titleIschemia-induced ribosomal protein S3 expressional changes and the neuroprotective effect against experimental cerebral ischemic damageen
dc.typeArticleen
dc.contributor.AlternativeAuthor황인구-
dc.contributor.AlternativeAuthor유기연-
dc.contributor.AlternativeAuthor김대원-
dc.contributor.AlternativeAuthor김소영-
dc.contributor.AlternativeAuthor박전홍-
dc.contributor.AlternativeAuthor류재영-
dc.contributor.AlternativeAuthor김준-
dc.contributor.AlternativeAuthor최수영-
dc.contributor.AlternativeAuthor원무호-
dc.identifier.doi10.1002/jnr.21621-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Neurology (신경과학교실)Journal Papers (저널논문_신경과학교실)
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