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Stretch-activated currents in cardiomyocytes isolated from rabbit pulmonary veins
DC Field | Value | Language |
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dc.contributor.author | Seol, Chang Ahn | - |
dc.contributor.author | Kim, Won Tae | - |
dc.contributor.author | Ha, Jeong Mi | - |
dc.contributor.author | Choe, Han | - |
dc.contributor.author | Jang, Yeon Jin | - |
dc.contributor.author | Youm, Jae Boum | - |
dc.contributor.author | Earm, Yung E | - |
dc.contributor.author | Leem, Chae Hun | - |
dc.date.accessioned | 2010-07-01T23:27:28Z | - |
dc.date.available | 2010-07-01T23:27:28Z | - |
dc.date.issued | 2008-03-21 | - |
dc.identifier.citation | Prog Biophys Mol Biol. 97(2-3), 217-231 | en |
dc.identifier.issn | 0079-6107 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18353429 | - |
dc.identifier.uri | http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6TBN-4RV7Y86-4-H&_cdi=5147&_user=168665&_orig=search&_coverDate=07%2F31%2F2008&_sk=999029997&view=c&wchp=dGLbVtb-zSkWz&md5=57629a6938c2bd328c68eb651dc83318&ie=/sdarticle.pdf | - |
dc.identifier.uri | https://hdl.handle.net/10371/68183 | - |
dc.description.abstract | Evidence is growing of a relationship between atrial dilation and atrial fibrillation (AF), the most prevalent type of arrhythmia. Pulmonary veins, which are important ectopic foci for provoking AF, are of increasing interest in relation to the early development of AF. Here, using single cardiomyocytes isolated from rabbit pulmonary veins, we characterised the stretch-activated currents induced by swelling and axial mechanical stretching. Swelling induced both a stretch-activated nonselective cationic current (NSC) and a Cl(-) current. The swelling-induced Cl(-) current (I Cl,swell) was inhibited by DIDS, whereas the swelling-induced NSC (I NSC,swell) was inhibited by Gd3+. The cationic selectivity of the I NSC,swell was K+ >Cs+ >Na+ >Li+, whilst the PK/PNa, PCs/PNa, and PLi/PNa permeability ratios were 2.84, 1.86, and 0.85, respectively. Activation of the I NSC,swell was faster than that of the I Cl,swell. Given a high K+ concentration in the bath solution, the I NSC,swell showed limited amplitude (<-70 mV). Mechanical stretching induced an immediate Gd3+- and streptomycin-sensitive NSC (I NSC,stretch) that was permeable to Na+, K+, Cs+ and NMDG. Persistent stretching activated a DIDS-sensitive current (I Cl,stretch). The I NSC,stretch, but not the I NSC,swell, was completely blocked by 400 microM streptomycin; therefore, the two currents may not be associated with the same channel. In addition, the type of current induced may depend on the type of stretching. Thus, stretch-induced anionic and cationic currents are functionally present in the cardiomyocytes of the main pulmonary veins of rabbits, and they may have pathophysiological roles in the development of AF under stretched conditions. | en |
dc.description.sponsorship | This work was supported by grant no. R01-2004-000-10374-0 from the KOSEF and was
supported by Korea Research Foundation Grant funded by Korea Government (MOEHRD, Basic Research Promotion Fund, KRF-2005-003-E00014). | en |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.subject | 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid/pharmacology | en |
dc.subject | Animals | en |
dc.subject | Atrial Fibrillation/*physiopathology | en |
dc.subject | Ion Channel Gating/drug effects/*physiology | en |
dc.subject | Ion Transport | en |
dc.subject | Ions/metabolism | en |
dc.subject | Mechanotransduction, Cellular/*physiology | en |
dc.subject | Membrane Potentials | en |
dc.subject | Myocytes, Cardiac/*physiology | en |
dc.subject | Pulmonary Veins/*cytology | en |
dc.subject | Rabbits | en |
dc.subject | Streptomycin/pharmacology | en |
dc.subject | Stress, Mechanical | en |
dc.title | Stretch-activated currents in cardiomyocytes isolated from rabbit pulmonary veins | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 설창안 | - |
dc.contributor.AlternativeAuthor | 김원태 | - |
dc.contributor.AlternativeAuthor | 하정미 | - |
dc.contributor.AlternativeAuthor | 최한 | - |
dc.contributor.AlternativeAuthor | 장연진 | - |
dc.contributor.AlternativeAuthor | 염재범 | - |
dc.contributor.AlternativeAuthor | 엄융의 | - |
dc.contributor.AlternativeAuthor | 임채훈 | - |
dc.identifier.doi | 10.1016/j.pbiomolbio.2008.02.008 | - |
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