Browse

Stretch-activated non-selective cation channel: a causal link between mechanical stretch and atrial natriuretic peptide secretion

Cited 16 time in Web of Science Cited 16 time in Scopus
Authors
Zhang, Yin Hua; Youm, Jae Boum; Earm, Yung E
Issue Date
2008-07-19
Publisher
Elsevier
Citation
Prog Biophys Mol Biol. 98 (1) 1-9
Keywords
AnimalsAtrial Natriuretic Factor/*metabolismBiological Assay*CationsElectrodesElectrophysiology/methodsEnzyme ActivationExocytosisHeart Atria/pathologyHumansModels, BiologicalPatch-Clamp TechniquesPeptides/chemistryRatsStress, Mechanical
Abstract
The polypeptide hormone atrial natriuretic peptide (ANP) plays vital roles in maintaining blood volume and arterial blood pressure. The recognition of clinical benefits of ANP both in healthy and diseased heart identifies ANP as a potential candidate for therapeutic strategy in the treatment of heart disease. ANP is synthesized and stored in cardiac myocytes and it is released through the exocytosis of ANP granules both constitutively and in response to stimuli. It is well known that mechanical stretch is the predominant stimulus for ANP secretion. However, the mechanistic link between mechanical stimuli and exocytosis of ANP vesicles in single atrial myocyte has not yet been demonstrated. Over the last decade, compelling evidence suggested that stretch-activated ion channels might function as mechanosensors. We showed previously that direct stretch of single atrial myocyte using two micro-electrodes activated a non-selective cation channel (SAC). So far it is not known whether activation of SAC is involved in stretch-induced ANP secretion. The present article aims to give an overview of the mechanism of mechanical stretch-stimulated ANP secretion and describes an innovative technique to detect ANP secretion from isolated rat atrial myocytes with high time-resolution. Combined with capacitance measurement and patch-clamp technique in conjunction with in situ ANP bioassay, we were able to demonstrate that SAC in rat atrial myocytes acts as a mechanosensor to transduce stretch signals into the ANP secretion pathway.
ISSN
0079-6107 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18635250

http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6TBN-4SMNXPG-1-1&_cdi=5147&_user=168665&_orig=search&_coverDate=09%2F30%2F2008&_sk=999019998&view=c&wchp=dGLzVzz-zSkWz&md5=a1b4ebdf3684c60f3451ff605c067b05&ie=/sdarticle.pdf

http://hdl.handle.net/10371/68190
DOI
https://doi.org/10.1016/j.pbiomolbio.2008.05.005
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Physiology (생리학교실)Journal Papers (저널논문_생리학교실)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse