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Label-free calcium imaging in ischemic retinal tissue by TOF-SIMS

Cited 19 time in Web of Science Cited 22 time in Scopus
Authors
Kim, Jin Hyoung; Kim, Jeong Hun; Ahn, Bum Ju; Park, Jae-Hwan; Shon, Hyun Kyong; Yu, Young Suk; Moon, Dae Won; Lee, Tae Geol; Kim, Kyu-Won
Issue Date
2008-01-30
Publisher
Elsevier
Citation
Biophys J. 94(10):4095-4102
Keywords
AnimalsCalcium/*metabolismIschemia/*metabolism/pathologyMaleMiceMice, Inbred C57BLRetinal Vessels/*metabolism/pathologySpectrometry, Mass, Electrospray Ionization/*methodsSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methodsStaining and LabelingTissue Distribution
Abstract
The distribution and movement of elemental ions in biologic tissues is critical for many cellular processes. In contrast to chemical techniques for imaging the intracellular distribution of ions, however, techniques for imaging the distribution of ions across tissues are not well developed. We used time-of-flight secondary ion mass spectrometry (TOF-SIMS) to obtain nonlabeled high-resolution analytic images of ion distribution in ischemic retinal tissues. Marked changes in Ca(2+) distribution, compared with other fundamental ions, such as Na(+), K(+), and Mg(2+), were detected during the progression of ischemia. Furthermore, the Ca(2+) redistribution pattern correlated closely with TUNEL-positive (positive for terminal deoxynucleotidyl transferase-mediated 2'-deoxyuridine 5'-triphosphate nick end-labeling) cell death in ischemic retinas. After treatment with a calcium chelator, Ca(2+) ion redistribution was delayed, resulting in a decrease in TUNEL-positive cells. These results indicate that ischemia-induced Ca(2+) redistribution within retinal tissues is associated with the order of apoptotic cell death, which possibly explains the different susceptibility of various types of retinal cells to ischemia. Thus, the TOF-SIMS technique provides a tool for the study of intercellular communication by Ca(2+) ion movement.
ISSN
1542-0086 (Electronic)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18227131

http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B94RW-4VB4SVB-18-1&_cdi=56421&_user=168665&_orig=search&_coverDate=05%2F15%2F2008&_sk=999059989&view=c&wchp=dGLbVlb-zSkzS&md5=4ca1bfdf754d48fc93511871024563e0&ie=/sdarticle.pdf

http://hdl.handle.net/10371/68213
DOI
https://doi.org/10.1529/biophysj.107.119800
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College of Medicine/School of Medicine (의과대학/대학원)Ophthalmology (안과학전공)Journal Papers (저널논문_안과학전공)
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