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Antiangiogenic effect of deguelin on choroidal neovascularization
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Jeong Hun | - |
dc.contributor.author | Kim, Jin Hyoung | - |
dc.contributor.author | Yu, Young Suk | - |
dc.contributor.author | Park, Kyu Hyung | - |
dc.contributor.author | Kang, Hye Jin | - |
dc.contributor.author | Lee, Ho-Young | - |
dc.contributor.author | Kim, Kyu-Won | - |
dc.date.accessioned | 2010-07-05 | - |
dc.date.available | 2010-07-05 | - |
dc.date.issued | 2007-10-31 | - |
dc.identifier.citation | J Pharmacol Exp Ther. 324(2):643-647 | en |
dc.identifier.issn | 1521-0103 (Electronic) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17967937 | - |
dc.identifier.uri | http://jpet.aspetjournals.org/content/324/2/643.full.pdf | - |
dc.identifier.uri | https://hdl.handle.net/10371/68233 | - |
dc.description.abstract | Age-related macular degeneration is the leading cause of blindness in the elderly. Choroidal neovascularization (CNV) leads to severe vision loss in patients of age-related macular degeneration. Previously, we have demonstrated that deguelin, isolated from plants in the Mundulea sericea family, is a chemopreventive agent. This study evaluates the antiangiogenic effect of deguelin on CNV. The toxicity of deguelin was evaluated through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay in human umbilical vein endothelial cells (HUVECs) as well as histological examination and terminal deoxynucleotidyl transferase dUTP nick-end labeling staining in the deguelin-injected retina. Antiangiogenic activity of deguelin was evaluated by in vitro tube formation assay of HUVECs and in vivo angiogenesis of chick chorioallantoic membrane (CAM). In C57BL/6 mice with laser-induced CNV, deguelin or phosphate-buffered saline was injected intravitreously. CNV lesions were examined by fluorescence angiography and vessel counting in cross-sections. Deguelin showed no effect on cell viability of HUVECs and no retinal toxicity in a concentration range of 0.01 to 1 microM. Deguelin effectively inhibited in vitro tube formation of HUVECs and in vivo angiogenesis of CAM. Interestingly, deguelin significantly reduced CNV and its leakage in mouse model of laser photocoagulation-induced CNV. Our data suggests that deguelin is a potent inhibitor of CNV and may be applied in the treatment of other vasoproliferative retinopathies such as retinopathy of prematurity and diabetic retinopathy. | en |
dc.language.iso | en | en |
dc.publisher | American Society for Pharmacology and Experimental Therapeutics | en |
dc.subject | Animals | en |
dc.subject | Cells, Cultured | en |
dc.subject | Choroid/blood supply/drug effects | en |
dc.subject | Choroidal Neovascularization/*drug therapy/pathology | en |
dc.subject | Female | en |
dc.subject | Humans | en |
dc.subject | Mice | en |
dc.subject | Mice, Inbred C57BL | en |
dc.subject | Rotenone/*analogs & derivatives/pharmacology/therapeutic use | en |
dc.title | Antiangiogenic effect of deguelin on choroidal neovascularization | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 김정훈 | - |
dc.contributor.AlternativeAuthor | 김진형 | - |
dc.contributor.AlternativeAuthor | 유영석 | - |
dc.contributor.AlternativeAuthor | 박규형 | - |
dc.contributor.AlternativeAuthor | 강혜진 | - |
dc.contributor.AlternativeAuthor | 이호영 | - |
dc.contributor.AlternativeAuthor | 김규원 | - |
dc.identifier.doi | 10.1124/jpet.107.132720 | - |
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