S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Neurology (신경과학교실) Journal Papers (저널논문_신경과학교실)
Neuroprotective effect of neural stem cell-conditioned media in in vitro model of Huntington's disease
- Lim, Heon-Chang; Lee, Soon-Tae; Chu, Kon; Joo, Kyung Min; Kang, Lami; Im, Woo-Seok; Park, Joung-Eun; Kim, Seung U; Kim, Manho; Cha, Choong-Ik
- Issue Date
- Neurosci Lett. 435 (2008) 175-180
- Apoptosis/drug effects; Cells, Cultured; Culture Media, Conditioned/*pharmacology; Dose-Response Relationship, Drug; Fetus; Flow Cytometry/methods; Humans; Nerve Tissue Proteins/genetics/metabolism; Neurons/chemistry/*drug effects; Neuroprotective Agents/*pharmacology; Nuclear Proteins/genetics; Stem Cells/*chemistry; Telencephalon/cytology; Transfection/methods; Trinucleotide Repeat Expansion/*genetics
- Although neural stem cell (NSC) transplantation has been investigated as a promising tool for reconstituting damaged brains, recent evidences suggest that NSCs may rescue the brain via paracrine effects rather than by direct cell replacements. In this study, we attempted to determine the neuroprotective effect of NSC-conditioned media (NSC-CM) in in vitro model of Huntington's disease. Cerebral hybrid neurons (A1) were transfected with either wild-type huntingtin (18 CAG repeats) or mutant huntingtin (100 CAG repeats). At 24h after the transfection, immunocytochemical patterns of the huntingtin aggregations, as well as the level of N-terminal proteolytic cleavages of huntingtin were analyzed. Neuronal apoptosis was evaluated with flowcytometry after Annexin-V and propidium iodide (PI) staining. Cerebral hybrid neurons transfected with mutant huntingtin showed five aggregates patterns, including diffuse cytoplasmic, dispered vacuoles, perinuclear vacuoles, nuclear inclusions (NI), and cytoplasmic inclusions (CI). NSC-CM reduced the levels of nuclear and cytoplasmic inclusions. The transfection with mutant huntingtin increased the level of N-terminal cleavages, which was reduced by the NSC-CM treatment. In addition, NSC-CM reduced the Annexin-V(+)PI(+) and Annexin-V(+)PI(-) neurons which were induced by the mutant huntingtin transfection. In summary, NSC-CM was neuroprotective in in vitro model of Huntington's disease with modulating mutant huntingtin-induced cytotoxicity.
- 0304-3940 (Print)
- Files in This Item: There are no files associated with this item.