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Inhibitory effects of flavonoids on HMG-CoA reductase and intestinal microflora growth
HMG-CoA reductase 활성과 장내 미생물 생육에 대한 플라보노이드의 영향

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Authors
전구주
Advisor
문태화
Issue Date
2003
Publisher
서울대학교 대학원
Keywords
HMG-CoA 리덕타제HMG-CoA reductase플라보노이드Flavonoids방사선 동위원소Radioisotope장내미생물Intestinal microflora16S rRNA16S rRNADNA상동성(Blastn)Blastn
Description
Thesis (master`s)--서울대학교 대학원 :농생명공학부,2003.
Abstract
The effects of flavonoids an inhibition of 3-hydroxy-3-methylglutaryl coenzyme A
(HMG-CoA) reductase, which is rate limiting enzyme in cholesterol biosynthesis,
and growth-inhibition of intestinal microflora were investigated. Syrian hamster
HMG-CoA reductase was overexpressed though the plasmid pKFT7-21 purified by Blue
Sepharose CL-6B, chromatography. The IC50 values of 29 Flavonoids (flavones,
flavanones, flavonols, flavanols, and isoflavones) were measured by modified
microradio assay with [14C] HMG-CoA, and Vero cells were used to confirm cell
proliferation. It was found that morin (2'', 3, 4'', 5, 7-pentahydroxyflavone)
has remarkable effect on inhibition of HMG-CoA reductase having IC50 value of
16.7 (¡¾1.4) uM. It, however, did not show a significant morphological reversion
of Vero cell at 100 mg/ml concentration. Regarding growth-inhibition of
intestinal microflora, Bifidobacterium animalis, Enterococcus faecalis,
Escherichia coli, and Clostridium perfringens were isolated with selective media
and identified by 16S rRNA sequence homologue. Effects on growth-inhibition of
intestinal harmful bacteria were determined by paper disks treated with one
milligram flavonoids. Catechin and epicatechin showed a remarkable effect and
morin was moderate for growth-inhibition against Cl. perfringens and E. coli,
but did not show significant effect on useful bacteria. Comparing with total
anaerobic intestinal bacteria at each morin concentration, relative composition
of Bifidobacterium anmalis increased in and that of Cl. perfingens decreased.
Language
English
URI
http://dcollection.snu.ac.kr:80/jsp/common/DcLoOrgPer.jsp?sItemId=000000057526

http://hdl.handle.net/10371/68532
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Theses (Master's Degree_식품·동물생명공학부)
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