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A role of sodium bicarbonate cotransporter(NBC) in HCO3- formation in human salivary gland acinar cells

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dc.contributor.authorJin, Meehyun-
dc.contributor.authorKoo, Na-Youn-
dc.contributor.authorJin, MeiHong-
dc.contributor.authorHwang, Sung-Min-
dc.contributor.authorPark, Kyungpyo-
dc.date.accessioned2010-07-12T04:36:02Z-
dc.date.available2010-07-12T04:36:02Z-
dc.date.issued2008-
dc.identifier.citationInternational Journal of Oral Biology, 2008;33(4):143-147en
dc.identifier.issn1226-7155-
dc.identifier.urihttp://hdl.handle.net/10371/68588-
dc.description.abstractThe sodium bicarbonate cotransporter (NBC) protein is functionally expressed in salivary glands. In this experiment, we examined the role of NBC in HCO3- formation in human parotid gland acinar cells. Intracellular pH (pHi) was measured in 2'-7'-bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF) -loaded cells. Acetazolamide (0.1 mM) and 4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid (DIDS, 0.5 mM) were used as specific inhibitors of carbonic anhydrase and NBC, respectively. The degree of inhibition was assessed by measuring the pHi recovery rate (△pHi/min) after cell acidification using an ammonium prepulse technique. In control experiments, △pHi/min was 1.40 ± 0.06. Treatment of cells with 0.5 mM DIDS or 0.1 mM acetazolamide significantly reduced △pH/min to 1.14 ± 0.14 and 0.74 ± 0.15, respectively. Simultaneous application of DIDS and acetazolamide further reduced △ pHi/min to 0.47 ± 0.10. Therefore, DIDS and acetazolamide reduced △pHi/min by 19 % and 47 %, respectively, while simultaneous application of both DIDS and acetazolamide caused a reduction in △pHi/min of 67 %. These results suggest that in addition to carbonic anhydrase, NBC also partially contributes to HCO3- formation in human parotid gland acinar cells.en
dc.description.sponsorshipThis work was supported by the Korea Science &
Engineering Foundation (KOSEF) grant funded by
Korea government (R13-2008-008-01001-0) through
the Oromaxillofacial Dysfunction Research Center for
the Elderly(ODRCE) at Seoul National University.
en
dc.language.isokoen
dc.publisherThe Korean Academy of Oral Biologyen
dc.subjectsodium bicarbonate cotransporter (NBC)en
dc.subjecthuman parotid glanden
dc.subjectpHi recoveryen
dc.subject4'-diisothio cyanatostilbene-2en
dc.subject2'-disulphonic acid (DIDS)en
dc.subjectacetazolamideen
dc.titleA role of sodium bicarbonate cotransporter(NBC) in HCO3- formation in human salivary gland acinar cellsen
dc.typeArticleen
dc.contributor.AlternativeAuthor진미현-
dc.contributor.AlternativeAuthor구나윤-
dc.contributor.AlternativeAuthor진미홍-
dc.contributor.AlternativeAuthor황성민-
dc.contributor.AlternativeAuthor박경표-
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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