Publications
Detailed Information
A role of sodium bicarbonate cotransporter(NBC) in HCO3- formation in human salivary gland acinar cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jin, Meehyun | - |
dc.contributor.author | Koo, Na-Youn | - |
dc.contributor.author | Jin, MeiHong | - |
dc.contributor.author | Hwang, Sung-Min | - |
dc.contributor.author | Park, Kyungpyo | - |
dc.date.accessioned | 2010-07-12T04:36:02Z | - |
dc.date.available | 2010-07-12T04:36:02Z | - |
dc.date.issued | 2008 | - |
dc.identifier.citation | International Journal of Oral Biology, 2008;33(4):143-147 | en |
dc.identifier.issn | 1226-7155 | - |
dc.identifier.uri | https://hdl.handle.net/10371/68588 | - |
dc.description.abstract | The sodium bicarbonate cotransporter (NBC) protein is functionally expressed in salivary glands. In this experiment, we examined the role of NBC in HCO3- formation in human parotid gland acinar cells. Intracellular pH (pHi) was measured in 2'-7'-bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF) -loaded cells. Acetazolamide (0.1 mM) and 4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid (DIDS, 0.5 mM) were used as specific inhibitors of carbonic anhydrase and NBC, respectively. The degree of inhibition was assessed by measuring the pHi recovery rate (△pHi/min) after cell acidification using an ammonium prepulse technique. In control experiments, △pHi/min was 1.40 ± 0.06. Treatment of cells with 0.5 mM DIDS or 0.1 mM acetazolamide significantly reduced △pH/min to 1.14 ± 0.14 and 0.74 ± 0.15, respectively. Simultaneous application of DIDS and acetazolamide further reduced △ pHi/min to 0.47 ± 0.10. Therefore, DIDS and acetazolamide reduced △pHi/min by 19 % and 47 %, respectively, while simultaneous application of both DIDS and acetazolamide caused a reduction in △pHi/min of 67 %. These results suggest that in addition to carbonic anhydrase, NBC also partially contributes to HCO3- formation in human parotid gland acinar cells. | en |
dc.description.sponsorship | This work was supported by the Korea Science &
Engineering Foundation (KOSEF) grant funded by Korea government (R13-2008-008-01001-0) through the Oromaxillofacial Dysfunction Research Center for the Elderly(ODRCE) at Seoul National University. | en |
dc.language.iso | ko | en |
dc.publisher | The Korean Academy of Oral Biology | en |
dc.subject | sodium bicarbonate cotransporter (NBC) | en |
dc.subject | human parotid gland | en |
dc.subject | pHi recovery | en |
dc.subject | 4'-diisothio cyanatostilbene-2 | en |
dc.subject | 2'-disulphonic acid (DIDS) | en |
dc.subject | acetazolamide | en |
dc.title | A role of sodium bicarbonate cotransporter(NBC) in HCO3- formation in human salivary gland acinar cells | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 진미현 | - |
dc.contributor.AlternativeAuthor | 구나윤 | - |
dc.contributor.AlternativeAuthor | 진미홍 | - |
dc.contributor.AlternativeAuthor | 황성민 | - |
dc.contributor.AlternativeAuthor | 박경표 | - |
- Appears in Collections:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.