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Calcium Hydroxide Inactivates Lipoteichoic Acid from Enterococcus faecalis

Cited 15 time in Web of Science Cited 15 time in Scopus
Authors
Baik, Jung Eun; Kum, Kee-Yeon; Yun, Cheol-Heui; Lee, Jin-Kyung; Lee, Kangseok; Kim, Kack Kyun; Han, Seung Hyun
Issue Date
2008-11
Publisher
Elsevier
Citation
J Endod. 2008;34(11):1355-1359
Keywords
Apical periodontitiscalcium hydroxideendodontic medicamentEnterococcus faecalislipoteichoic acidToll-like receptor 2
Abstract
Calcium hydroxide is a widely used endodontic medicament for eliminating viable bacteria and inactivating virulence factors. Enterococcus faecalis, a pathogenic gram-positive bacterium, has been associated with refractory apical periodontitis. Because lipoteichoic acid (LTA) is a major virulence factor of gram-positive bacteria, we examined whether calcium hydroxide could detoxify LTA from E. faecalis. An enzyme-linked immunosorbent assay showed that calcium hydroxide–killed E. faecalis was less potent than heat-killed bacteria in stimulating the release of tumor necrosis factor–α by a murine macrophage line, RAW 264.7 (P < 0.05). Pretreatment of LTA with calcium hydroxide remarkably abrogated the ability of LTA to induce the release of tumor necrosis factor–α (P < 0.05). Furthermore, calcium hydroxide–treated LTA was not able to stimulate Toll-like receptor 2, which recognizes functionally intact LTA. These results suggest that calcium hydroxide could detoxify LTA, resulting in attenuation of the inflammatory responses to E. faecalis and its LTA.
ISSN
0099-2399
Language
English
URI
http://hdl.handle.net/10371/68597
DOI
https://doi.org/10.1016/j.joen.2008.08.014
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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