Publications
Detailed Information
Calcium Hydroxide Inactivates Lipoteichoic Acid from Enterococcus faecalis
Cited 19 time in
Web of Science
Cited 19 time in Scopus
- Authors
- Issue Date
- 2008-11
- Publisher
- Elsevier
- Citation
- J Endod. 2008;34(11):1355-1359
- Keywords
- Apical periodontitis ; calcium hydroxide ; endodontic medicament ; Enterococcus faecalis ; lipoteichoic acid ; Toll-like receptor 2
- Abstract
- Calcium hydroxide is a widely used endodontic medicament for eliminating viable bacteria and inactivating virulence factors. Enterococcus faecalis, a pathogenic gram-positive bacterium, has been associated with refractory apical periodontitis. Because lipoteichoic acid (LTA) is a major virulence factor of gram-positive bacteria, we examined whether calcium hydroxide could detoxify LTA from E. faecalis. An enzyme-linked immunosorbent assay showed that calcium hydroxide–killed E. faecalis was less potent than heat-killed bacteria in stimulating the release of tumor necrosis factor–α by a murine macrophage line, RAW 264.7 (P < 0.05). Pretreatment of LTA with calcium hydroxide remarkably abrogated the ability of LTA to induce the release of tumor necrosis factor–α (P < 0.05). Furthermore, calcium hydroxide–treated LTA was not able to stimulate Toll-like receptor 2, which recognizes functionally intact LTA. These results suggest that calcium hydroxide could detoxify LTA, resulting in attenuation of the inflammatory responses to E. faecalis and its LTA.
- ISSN
- 0099-2399
- Language
- English
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.