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Induction of Bone Morphogenetic Protein-2 from Gingival Epithelial Cells by Oral Bacteria

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dc.contributor.authorChoi, Youngnim-
dc.contributor.authorKim, Youngsook-
dc.contributor.authorJi, Suk-
dc.contributor.authorJung, Hong-Moon-
dc.contributor.authorWoo, Kyung-Mi-
dc.date.accessioned2010-07-23-
dc.date.available2010-07-23-
dc.date.issued2007-09-
dc.identifier.citationInternational Journal of Oral Biology, 32 : 103-107en
dc.identifier.issn1226-7155-
dc.identifier.urihttp://hdl.handle.net/10371/68664-
dc.description.abstractWe hypothesized that plaque-associated bacteria may
have a role in maintenance of alveolar bone. To test it,
immortalized gingival epithelial HOK-16B cells were cocultured
with live or lysed eight plaque bacterial species and
the expression levels of bone morphogenetic protein (BMP)-
2 and -4 were examined by real time reverse transcriptionpolymerase
chain reaction. Un-stimulated HOK-16B cells
expressed both BMP-2 and -4. Co-culture with plaque
bacterial lysates had significant effects on the level of BMP-
2 but not on that of BMP-4. Five species including
Streptococcus sanguinis, S. gordonii, Veillonella atypica,
Porphyromonas gingivalis, and Treponema denticola substantially
up-regulated the level of BMP-2. In contrary to
the upregulatory effect of lysate, live T. denticola suppressed
the expression of BMP-2. In addition, in vitro osteoblastic
differentiation assay using C2C12 cells and the conditioned
medium of HOK-16B cells confirmed the production of
BMPs by gingival epithelial cells and the modulation of
BMP expression by the lysates of S. sanguinis and T.
denticola. In conclusion, we have shown that plaque bacteria
can regulate the expresion of BMP-2 by gingival epithelial
cells, the physiologic meaning of which needs further
investigation.
en
dc.language.isoenen
dc.publisherKorean Acadamy of Oral Biologyen
dc.subjectoral bacteriaen
dc.subjectgingival epitheliumen
dc.subjectBMPen
dc.subjectosteoblasten
dc.titleInduction of Bone Morphogenetic Protein-2 from Gingival Epithelial Cells by Oral Bacteriaen
dc.typeArticleen
dc.contributor.AlternativeAuthor최영님-
dc.contributor.AlternativeAuthor김영숙-
dc.contributor.AlternativeAuthor지숙-
dc.contributor.AlternativeAuthor정홍문-
dc.contributor.AlternativeAuthor우경미-
Appears in Collections:
College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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