Browse

Regulating the role of bone morphogenetic protein 4 in tooth bioengineering

Cited 6 time in Web of Science Cited 6 time in Scopus
Authors
Choung, Pill-Hoon; Chung, Il-Hyuk; Ryu, Hyun-Jeong; Kang, Young-Ho; Choung, Han-Wool; Chung, Jong-Hoon; Choung, Yun-Hoon
Issue Date
2007-03
Publisher
Elsevier
Citation
Journal of Oral and Maxillofacial Surgery 65:501-507
Abstract
Purpose
Culture of the whole organ and regulation of its development using biologic and engineering principles can be used to produce structures and organs for reconstructing defects. The application of these bioengineering approaches in artificial tooth development may be the alternative way to replace missing dentition.

Materials and Methods
For the artificial bioengineering of a mouse tooth, tooth buds were dissected and transplanted into the diastema of the developing mandible. The mandiblular primordia containing transplanted tooth buds were culture in vitro and in vivo using a bioengineering method. In addition, to regulate the development of tooth germs, bone morphogenetic protein 4 (BMP4) or its antagonist, Noggin was administered.

Results
After the period of in vitro and in vivo culture, the transplanted tooth germ in the diastema showed tooth development with supportive structure formation. In the BMP-treated group, the bioengineered tooth was observed with increased maturation of cusp and enamel matrix. However, in the Noggin-treated tooth germs, the developing molar had a crater-like appearance with the immature development of the cusp and suppressed formation of the enamel matrix.

Conclusions
This study confirmed that tooth germ transplantation in the diastema and culture with administration of BMP4 could lead to the mature development of the dental structures. In addition, these results suggest the possibility of bioengineering the tooth in morphogenesis and differentiation even in the toothless area.
ISSN
0278-2391
Language
English
URI
http://hdl.handle.net/10371/68703
DOI
https://doi.org/10.1016/j.joms.2006.07.004
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse