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B cells activated in the presence of Th1 cytokines inhibit osteoclastogenesis.
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Youngnim | - |
dc.contributor.author | Kim, Jeong Jae | - |
dc.date.accessioned | 2010-08-02T01:11:54Z | - |
dc.date.available | 2010-08-02T01:11:54Z | - |
dc.date.issued | 2003-10 | - |
dc.identifier.citation | Exp Mol Med. 2003;35(5):385-392 | en |
dc.identifier.issn | 1226-3613 | - |
dc.identifier.uri | https://hdl.handle.net/10371/68833 | - |
dc.description.abstract | Host immune response has been considered as an important disease-modifying factor of periodontitis, however, which immune cell(s) or factor(s) are involved in the destruction of periodontium remains unclear. Previously, we reported that osteoclastogenesis is enhanced by activated B cells but suppressed by activated CD8+ T cells. We present new data that B cells activated in the presence of Th1 cytokines inhibit osteoclastogenesis. Purified murine B cells were activated with anti-IgD mAb, IL-4, and anti-CD40 mAb, in the absence (BTh2) or presence of Th1 cytokines, either IL-2 (BIL-2) or IFN-gamma (BIFN-gamma). Each activated B cell population was co-cultured with RAW264.7 cells in the presence of soluble receptor activator of NF-kappaB ligand (sRANKL), and the effect on osteoclastic differentiation was evaluated. While BTh2 increased osteoclastogenesis, BIL-2 and BIFN-gamma suppressed it profoundly. To verify the mediating molecule(s), we analyzed cytokine profiles of the activated B cells. Compared to BTh2, BIL-2 expressed increased amount of IFN-gamma and BIFN-gamma expressed decreased amounts of IL-4, IL-5, and IL-10. IFN-gamma was a key negative regulator of osteoclastic differentiation, and mediated the inhibition by BIL-2. These results suggest that Th1 cytokines may have new important roles in resistance to periodontitis, acting directly on osteoclasts or indirectly through B cells. | en |
dc.description.sponsorship | This study was supported by Korea Research Foundation
grant 2000-041-F00276. The authors thank Dr. Jennifer M. Puck for the critical reading of manuscript. | en |
dc.language.iso | en | en |
dc.publisher | Korean Society of Medical Biochemistry | en |
dc.subject | B lymphocyte subsets | en |
dc.subject | cytokines | en |
dc.subject | interferon type II | en |
dc.subject | interleukin-2 | en |
dc.subject | osteoclasts | en |
dc.subject | Th1 cells | en |
dc.title | B cells activated in the presence of Th1 cytokines inhibit osteoclastogenesis. | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 최영님 | - |
dc.contributor.AlternativeAuthor | 김정재 | - |
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