SHERP

단백질칩 제작을 위한 유리표면의 고분자 개질화 및 DNA칩 제작을 위한 금전극 표면 개질화에 관한 연구

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Authors
장기훈
Advisor
이윤식
Issue Date
2003
Publisher
서울대학교 대학원
Keywords
단백질칩Protein chip비특이적 흡착Nonspecific binding고분자 표면Polymer-grafted surfaceMMAGold surface전기화학적 측정Electrochemical detectionDNA칩DNABifunctional linkerCV measurement
Description
학위논문(석사)--서울대학교 대학원 :응용화학부,2003.
Abstract
Nonspecific binding on a chip surface is very serious problem in a
protein chip fabrication. The effect of a polymer-grafted surface for
reducing nonspecific binding was observed by making protein-patterned
surface on a chip Firstly, the glass surface was epoxy-functionalized
with GPTS (3-Glycidoxypropyltrimethoxy silane) and coupled with
hydrophilic polymer such as chitosan and aminodextran. Then,
hydrphobic or hydrophilic spacer with a photolabile protecting group,
NVOC-ACA or NVOC-PEG, were coupled to the surface to ensure
proper interaction of a ligand with a protein on the chip surface. The
NVOC-protected surface was selectively irradiated by a UV illuminator
using micro mirror array. After removing the NVOC group, biotin was
coupled to the NVOC-cleaved site and incubated with
FITC/Cy3-conjugated streptavidin to obtain protein patterning, which
showed the effect of polymer-grafted surface in reducing nonspecific
binding.
Gold surfaces were modified for electrochemical detection of DNA on
a chip. To reduce nonspecific binding, PEG containing thiol spacer was
immobilized on a gold electrode surface. After bifunctional linker was
coupled to the spacer thiol modified oligonucleotide was immobilized.
Hybridization process was successfully detected by probe DNA and
methylene blue by CV measurement.
Language
Korean
URI
http://dcollection.snu.ac.kr:80/jsp/common/DcLoOrgPer.jsp?sItemId=000000058873

http://hdl.handle.net/10371/68921
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Theses (Master's Degree_화학생물공학부)
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