단백질칩 제작을 위한 유리표면의 고분자 개질화 및 DNA칩 제작을 위한 금전극 표면 개질화에 관한 연구

Abstract

Nonspecific binding on a chip surface is very serious problem in a protein chip fabrication. The effect of a polymer-grafted surface for reducing nonspecific binding was observed by making protein-patterned surface on a chip Firstly, the glass surface was epoxy-functionalized with GPTS (3-Glycidoxypropyltrimethoxy silane) and coupled with hydrophilic polymer such as chitosan and aminodextran. Then, hydrphobic or hydrophilic spacer with a photolabile protecting group, NVOC-ACA or NVOC-PEG, were coupled to the surface to ensure proper interaction of a ligand with a protein on the chip surface. The NVOC-protected surface was selectively irradiated by a UV illuminator using micro mirror array. After removing the NVOC group, biotin was coupled to the NVOC-cleaved site and incubated with FITC/Cy3-conjugated streptavidin to obtain protein patterning, which showed the effect of polymer-grafted surface in reducing nonspecific binding. Gold surfaces were modified for electrochemical detection of DNA on a chip. To reduce nonspecific binding, PEG containing thiol spacer was immobilized on a gold electrode surface. After bifunctional linker was coupled to the spacer thiol modified oligonucleotide was immobilized. Hybridization process was successfully detected by probe DNA and methylene blue by CV measurement.