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Proteomic profile of osteoclast membrane proteins: Identification of Na+/H+ exchanger domain containing 2 and its role in osteoclast fusion

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dc.contributor.authorRyoo, Hyun-Mo-
dc.contributor.authorHa, Byung Geun-
dc.contributor.authorHong, Jung Min-
dc.contributor.authorPark, Ju-Yong-
dc.contributor.authorHa, Mi-Hyun-
dc.contributor.authorKim, Tae-Ho-
dc.contributor.authorCho, Je-Yeol-
dc.contributor.authorChoi, Je-Yong-
dc.contributor.authorShin, Hong-In-
dc.contributor.authorChun, So Young-
dc.contributor.authorKim, Shin-Yoon-
dc.contributor.authorPark, Eui Kyun-
dc.date.accessioned2010-08-04T23:04:07Z-
dc.date.available2010-08-04T23:04:07Z-
dc.date.issued2008-07-
dc.identifier.citationProteomics 8, 2625-2639en
dc.identifier.issn1615-9853-
dc.identifier.urihttp://hdl.handle.net/10371/68971-
dc.description.abstractOsteoclast formation and bone resorption are multiple processes that involve the participation of specialized membrane structures and their associated proteins. In this study, we used an MS to analyze the profile of proteins associated with osteoclast membranes and focused on the function of channel proteins in osteoclast differentiation and function. We filtered out with a SEQUEST score greater than 10 and a peptide hit number of more than 2, resulting in the identification of 499 proteins that were commonly found in both macrophages and osteoclasts, 96 proteins selectively found in osteoclasts, and 179 proteins selectively found in macrophages. The proteins that were selectively found in osteoclasts were classified based on their localizations: plasma membrane (17%), ER/Golgi and lysosome/endosome (15%), mitochondrion (18%), nucleus (13%), cytosol (19%), and unknown (18%). Proteins associated with osteoclast function such as v-ATPase, IGF2R, TRAP, and cathepsin K were found in osteoclasts as previously shown. We found several ion channel proteins such as Ank and Nhedc2 and signaling molecules such as Dock5 and RAB-10 in osteoclasts. Inhibition of the Na+/H+ exchanger family by amiloride suppressed RANKL-induced osteoclast fusion and bone resorption. In addition, shRNA for Nhedc2 inhibited osteoclast differentiation. Our results provide a proteomic profile of osteoclast membrane proteins and identify Nhedc2, which is probably associated with proton transport in osteoclasts, as a regulator of osteoclast function.en
dc.description.sponsorshipThe work was supported in part by the grants of the Korea
Health 21 R&D Project (Ministry of Health,Welfare and Family
Affairs, Republic of Korea, A010252 and A030003) and in part
by a Kyungpook National University Hopsital Biomedical Research
Institute Grant (2004).
en
dc.language.isoenen
dc.publisherWiley-Blackwellen
dc.subjectMacrophagesen
dc.subjectMembrane proteinsen
dc.subjectOsteoclastsen
dc.subjectRANKLen
dc.titleProteomic profile of osteoclast membrane proteins: Identification of Na+/H+ exchanger domain containing 2 and its role in osteoclast fusionen
dc.typeArticleen
dc.contributor.AlternativeAuthor류현모-
dc.contributor.AlternativeAuthor하병근-
dc.contributor.AlternativeAuthor홍정민-
dc.contributor.AlternativeAuthor박주영-
dc.contributor.AlternativeAuthor하미현-
dc.contributor.AlternativeAuthor김태호-
dc.contributor.AlternativeAuthor조재열-
dc.contributor.AlternativeAuthor최재영-
dc.contributor.AlternativeAuthor신홍인-
dc.contributor.AlternativeAuthor전수영-
dc.contributor.AlternativeAuthor김신연-
dc.contributor.AlternativeAuthor박유경-
dc.identifier.doi10.1002/pmic.200701192-
Appears in Collections:
College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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