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Runx2 phosphorylation induced by fibroblast growth factor-2/protein kinase C pathways

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dc.contributor.authorRyoo, Hyun-Mo-
dc.contributor.authorKim, Byung-Gyu-
dc.contributor.authorKim, Hyun-Jung-
dc.contributor.authorPark, Hye-Jeong-
dc.contributor.authorKim, Youn-Jeong-
dc.contributor.authorYoon, Won-Joon-
dc.contributor.authorLee, Seung-Jin-
dc.contributor.authorCho, Je-Yoel-
dc.date.accessioned2010-09-03T06:02:13Z-
dc.date.available2010-09-03T06:02:13Z-
dc.date.issued2006-01-
dc.identifier.citationProteomics 2006, 6, 1166-1174en
dc.identifier.issn1615-9853-
dc.identifier.urihttp://hdl.handle.net/10371/69663-
dc.description.abstractRunx2 is a key transcription factor in osteoblast differentiation, and its activity is regulated by fibroblast growth factors (FGFs). Craniosynostosis, characterized by premature suture closure, results from mutations that generate constitutively active FGF receptors (FGFRs). We previously showed that FGF/FGFR-activated protein kinase C (PKC) is involved in the expression and activity of Runx2. Activated PKC physically interacts with Runx2 in FGF2-stimulated MC3T3-E1 preosteoblastic cells. Immunopurified Runx2 protein reacted with PKC kinase, and a phosphorylated 1460-Da peptide fragment (amino acids 241-252, 1380-Da) derived from Runx2 was also detected in MS analysis. Computer analysis predicted that Ser247 in this Runx2 can be a possible phosphorylation site by PKC. We also showed that Runx2 activity after FGF stimulation correlates with the presence of the Runx2 Ser247 residue. The S247A (Ser Ala) mutation confers decreased transcriptional activity on a Runx2-responsive promoter after FGF treatment.en
dc.language.isoenen
dc.publisherWiley-Blackwellen
dc.subjectFibroblast growth factoren
dc.subjectFGF receptoren
dc.subjectMALDI-TOFen
dc.subjectPhosphorylationen
dc.subjectProtein kinase Cen
dc.subjectRunx2en
dc.titleRunx2 phosphorylation induced by fibroblast growth factor-2/protein kinase C pathwaysen
dc.typeArticleen
dc.contributor.AlternativeAuthor류현모-
dc.contributor.AlternativeAuthor김병규-
dc.contributor.AlternativeAuthor김현정-
dc.contributor.AlternativeAuthor박혜정-
dc.contributor.AlternativeAuthor김연정-
dc.contributor.AlternativeAuthor연원준-
dc.contributor.AlternativeAuthor이승진-
dc.contributor.AlternativeAuthor조재열-
dc.identifier.doi10.1002/pmic.200500289-
Appears in Collections:
College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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