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Birth of viable female dogs produced by somatic cell nuclear transfer

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Authors
Jang, Goo; Kim, M.K.; Oh, H.J.; Hossein, M.S.; Fibrianto, Y.H.; Hong, S.G.; Park, J.E.; Kim, J.J.; Kim, H.J.; Kang, S.K.; Kim, Dae-Yong; Lee, Byeong Chun
Issue Date
2006-12-13
Publisher
Elsevier
Citation
Theriogenology 2007;67:941-947
Keywords
Female cloned dogsSCNTIn vivo oocyteEmbryo transferPregnancy
Abstract
Since the only viable cloned offspring born in dogs was a male, the purpose of the present study was to produce female puppies by somatic cell nuclear transfer (SCNT). Adult ear fibroblasts from a 2-month-old female Afghan hound were isolated and used as donor cells. In vivo-matured canine oocytes surgically collected (approximately 72 h after ovulation) from the oviducts of 23 donors were used for SCNT. After removal of the cumulus cells, oocytes were enucleated, microinjected, fused with a donor cell, and activated. A total of 167 reconstructed SCNT embryos were surgically transferred (Day 0) into the oviducts of 12 recipient bitches (average 13.9 embryos/recipient, range 6–22) with spontaneous, synchronous estrous cycles. Three pregnancies were detected by ultrasonography on Day 23, maintained to term, and three healthy female puppies (520, 460, and 520 g), were delivered by Caesarean section on Day 60. These puppies were phenotypically and genotypically identical to the cell donor. In conclusion, we have provided the first demonstration that female dogs can be produced by nuclear transfer of ear fibroblasts into enucleated canine oocytes.
ISSN
0093-691X
Language
English
URI
http://hdl.handle.net/10371/6973
DOI
https://doi.org/10.1016/j.theriogenology.2006.11.006
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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