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Gateway RFP-fusion vectors for high-throughput functional analysis of genes
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Jae-Yong | - |
dc.contributor.author | Hwang, Eun Mi | - |
dc.contributor.author | Park, Nammi | - |
dc.contributor.author | Kim, Eunju | - |
dc.contributor.author | Kim, Dong-Gyu | - |
dc.contributor.author | Kang, Dawon | - |
dc.contributor.author | Han, Jaehee | - |
dc.contributor.author | Choi, Wan Sung | - |
dc.contributor.author | Ryu, Pan Dong | - |
dc.contributor.author | Hong, Seong-Geun | - |
dc.date.accessioned | 2009-08-12T07:40:20Z | - |
dc.date.available | 2009-08-12T07:40:20Z | - |
dc.date.issued | 2007 | - |
dc.identifier.citation | Mol. Cells 23, 357-362 | en |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.uri | http://molcells.inforang.com/about/journal_view.html?uid=257 | - |
dc.identifier.uri | https://hdl.handle.net/10371/7031 | - |
dc.description.abstract | There is an increasing demand for high throughput
(HTP) methods for gene analysis on a genome-wide scale. However, the current repertoire of HTP detection methodologies allows only a limited range of cellular phenotypes to be studied. We have constructed two HTP-optimized expression vectors generated from the red fluorescent reporter protein (RFP) gene. These vectors produce RFP-tagged target proteins in a multiple expression system using gateway cloning technology (GCT). The RFP tag was fused with the cloned genes, thereby allowing us localize the expressed proteins in mammalian cells. The effectiveness of the vectors was evaluated using an HTP-screening system. Sixty representative human C2 domains were tagged with RFP and overexpressed in HiB5 neuronal progenitor cells, and we studied in detail two C2 domains that promoted the neuronal differentiation of HiB5 cells. Our results show that the two vectors developed in this study are useful for functional gene analysis using an HTP-screening system on a genome-wide scale. | en |
dc.description.sponsorship | We appreciate the helpful advice of Dr. Tobias
Meyer and Dr. Won Do Heo of Stanford University in the construction of the set of entry clones of human C2 domains. This work was supported by a grant from the Basic Research Program of the Korea Science and Engineering Foundation (R01-2002- 000-00128-0), and a Korea Research Foundation Grant (KRF- 2006-005-J04204). | en |
dc.language.iso | en | - |
dc.publisher | 한국분자·세포생물학회 = Korean Society of Molecular and Cellular Biology | en |
dc.subject | Gateway Cloning System | en |
dc.subject | Red Fluorescent Protein | en |
dc.subject | High Throughput Screening System | en |
dc.title | Gateway RFP-fusion vectors for high-throughput functional analysis of genes | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 박재용 | - |
dc.contributor.AlternativeAuthor | 황은미 | - |
dc.contributor.AlternativeAuthor | 박남미 | - |
dc.contributor.AlternativeAuthor | 김은주 | - |
dc.contributor.AlternativeAuthor | 김동규 | - |
dc.contributor.AlternativeAuthor | 강다원 | - |
dc.contributor.AlternativeAuthor | 한재희 | - |
dc.contributor.AlternativeAuthor | 최완성 | - |
dc.contributor.AlternativeAuthor | 류판동 | - |
dc.contributor.AlternativeAuthor | 홍성근 | - |
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