Browse

Immunoproteomic analysis of capsulate and non-capsulate strains of Lactococcus garvieae

Cited 17 time in Web of Science Cited 18 time in Scopus
Authors
Shin, G.W.; Palaksha, K.J.; Kim, Y.R.; Nho, S.W.; Cho, J.H.; Heo, N.E.; Heo, G.J.; Park, Se Chang; Jung, T.S.
Issue Date
2006-08-26
Publisher
Elsevier
Citation
Vet. Microbiol. 119, 205-212
Keywords
Lactococcus garvieaeImmunoproteomicsCapsuleAntigenic profiles
Abstract
A comparative immunoproteomic study was carried out to investigate the immunogenicity of capsulate (KG9408) and non-capsulate (NSS9310) strains of Lactococcus garvieae. Immunoblot assays, following two-dimensional gel electrophoresis (2-DE) for L. garvieae strains, revealed a significant difference between anti-capsulate and anti-non-capsulate rabbit sera with respect to the number and antigenicity of antigenic spots. Anti-capsulate and anti-non-capsulate rabbit sera reacted with an average of 72 and 127 antigenic spots, respectively. The strong reaction of anti-non-capsulate sera with elongation factor (EF)-G and -Tu, and GMP synthase, of the L. garvieae strains identifies these as specific major antigens. This study clearly demonstrates the differences in 2-DE immunoblot profiles between the capsulate and non-capsulate strains of L. garvieae. These differences may be the reason for variations in immunogenicity between capsulate and non-capsulate strains. Glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase, arginine deaminase and ornithine carbamoyltransferase were identified from the 2-DE immunoblot profiles of both strains. Therefore, these common antigens are potential markers for the development of vaccines against L. garvieae, irrespective of strain. Immunoproteomics, a powerful tool for studying antigens at the proteomic level, allowed a comparative investigation of the immunogenicity of capsulate and non-capsulate strains of L. garvieae for vaccine development.
ISSN
0378-1135
Language
English
URI
http://hdl.handle.net/10371/7045
DOI
https://doi.org/10.1016/j.vetmic.2006.08.021
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse