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Molecular detection of betanodavirus in wild marine fish populations in Korea

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Authors
Gomez, Dennis Kaw; Baeck, Gun Wook; Kim, Ji Hyung; Choresca Jr, Casiano Hermopia; Park, Se Chang
Issue Date
2008
Publisher
American Association of Veterinary Laboratory Diagnosticians
Citation
J Vet Diagn Invest 20:38-44
Keywords
Betanodavirusphylogenetic analysispolymerase chain reaction–based techniquessubclinical infectionviral nervous necrosiswild fish
Abstract
Viral nervous necrosis (VNN) is a worldwide disease affecting several species of cultured marine fish. In Korea, VNN has been identified in several species of cultured marine fish. In this study, the authors present data of the amplified nested polymerase chain reaction product (420 bp) of 21 nodavirus strains from different species of apparently healthy wild marine fish on the southern coast of Korea. Phylogenetic analysis based on the partial nucleotide sequence (177 bases) of the RNA2 coat protein gene of 21 strains was highly homologous (93–100%) and closely related to that of the known betanodavirus, redspotted grouper nervous necrosis virus. These results indicate that betanodaviruses occur in large populations of wild marine fish in the southern part of the Korean peninsula, suggesting the importance of these subclinically infected fish as an inoculum source of betanodavirus that is horizontally transmitted to susceptible cultured fish species.
ISSN
1040-6387
Language
English
URI
http://jvdi.org/cgi/content/abstract/20/1/38

http://hdl.handle.net/10371/7049
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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