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Guanine Aminohydrolase 활성의 신비색측정법
A New Colorimetric Method for the Determination of Guanine Aminohydrolase Activity

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Authors
박주배; 김승원
Issue Date
1976-06
Publisher
서울대학교 의과대학
Citation
Seoul J Med 1976;17(2):136-142
Abstract
A new colorimetric assay method for guanine
deaminase was reported, coupling the enzyme
with xanthine oxidase and formazan
reaction system adding phenazine methosulfate
and INT (2-(p-iodophenyl)-3-(p-nitrophemyl)
-5-phenyl tetrazolium chloride). The method
was proved to be applicable both for the assay
of GOA activity and for visualization of the
enzymogram after electrophoresis.
The assay system consisted of GDA, XO as
much as 20 fold of GOA, O. 161'mole of PMS,
and 1. 51' mole of INT in a final volume of
3.0 ml. Tris buffer, pH 8. O. The reaction was
started by the addition of 1. 5 I' mole guanine,
followed by 30 minutes incubation at 30°C.
The present data was inconsistent with the
reported data, but could be converted by a
factor for the purpose of comparison. It appears
to be a good merit to apply the present
method in estimating the GOA activity after
staining electrophorogram with the formazan
reaction introduced in the present study directly
utilizing extract of visual enzyme band.
Language
English
URI
http://hdl.handle.net/10371/7180
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Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 17 No.2 (1976)
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