시구하부에 있어서 저장성 삼투압자극에 의하여 가토의 혈청에 이뇨작용이 출현하는 부위에 관한 실험적 연구

Abstract

Many studies have been reported on the role of the hypothalamus as the regulating center of the water and electrolyte balance. Since 1962. Rie and his associates studied on the effects of osmotic stimulations upon urine volume in various areas of the brain and demonstrated that the 3 areas in the cortex. 6 areas in the thalamus and hypothalamus and 6 areas in the midbrain showed osmoreceptic function. In 1972 Rie also studied on the effect of the serum of the rabbits derived 10 min and 30 min after injection of H20 into the anterior hypothalamus upon urine volume. and assumed that prompt and marked increase of urine volume following the injection of H20 into the above area was not because of inhibition of release of ADH or prompt depletion of ADH in the circulation but another factor. possibly due to diuretic hormone. The object of this study is to investigate the diu-retic effect of serum derived after osmotic stimulations in the various areas of hypothalamus upon urine volume of hydrated rabbits. Korean domestic white male rabbits weighing 1. 8 kg were used for this work. They were well hydrated oraly by giving 100 cc of 2% NaCI solution three times at one hour interval to maintain positive water balance through each study. The sites of osmotic stimulations were 6 areas of hypothalamus in where osmoreceptic functions were demonstrated in previous studies. 6 areas of hypothalamus were as following: 1) lower part of fornix in medial preoptic (area C in Fig. 1-1) 2) lower area of dorsal part of lateral geniculate body. (area G in Fig. 1-2) 3) habenulo-interpeduncular tract (area J in Fig. 1-2) 4) a little outside of paraventricular nucleus (area N in Fig. 1-2) 5) dorsal part of lateral geniculate body (area Q in Fig. 1-3) 6) lower part of medial geniculate body (area Cin Fig. 1-4) Into the above 6 areas of hypothalamus. osmotic stimulations were applied with distilled water and 0.9% NaCI solution separately using sterotaxic apparatus. 10 min and 30 min later, 1. 0 ml serum was obtained respectively. When the urine volume of another hydrated rabbits exceeded 4ml per 5 min. each 1.0 ml aove serum was given intravenously and observed changes of urine volume per 5min for over 150 min. The followings are observed: 1. Marked and prompt increase of urine volume was noted in the hydrated rabbit by the I\' injection of 1.0 ml serum derived 10 min after hypotonic osmotic stimulation into the C area of hypothalamus and the other hand diuretic activity of the serum derived 30 min after hypotonies t imulation as above was mild. 2. Also mild diuretic activity Was noted in the serum derived 10 min after hydotonic osmotic stimulation in the N area and ( area of hypothalamus. 3. No diuretic activity was observed in the serum derived 10 min and 30 min after hypotonic stimulation into the G area, J area and Q area of hypoth alamus. 4. Also there are no diuretic activity in the serums derived 10 min and 30 min after isotonic osmotic stimulation into the all 6areas of hypothalamus. Judging from the results of these experiments, the C, Nand, area of hypothalamus have the ability to produce some potent diuretic substance, probably diuretic hormone in the serum and itwas the most prominent in the C area.