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Sectioning and Scanning Electron Microscopy for Studies on the Embryonic Development

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Authors
Seo, Jeong Wook; Lee, Suk Keun; Kim, Eul Kyung; Wessels, Andy; Brown, Nigel A.
Issue Date
1994-12
Publisher
Seoul National University College of Medicine
Citation
Seoul J Med 1994;35(4):255-263
Keywords
Embryologysectional morphologyscanning electron microscopy
Abstract
A new method for an examination of the sectional plane with a
simultaneous visualization of the three dimensional morphology is introduced. which
is a combination of two classical methods; reconstruction of serially sectioned slides
and scanning electron microscopy.
Sectional surface was made either by serial step sectioning of an agar block using
vibratome, or by cryosectioning of a frozen specimen in gelatinous media. Some
histological techniques could be applied on the sections obtained and the rest of the
specimens were studied by scanning electron microscopy.
Step sectioning of an agar block was more useful for a routine screening procedure
and selected slices were processed further to the conventional histological sectioning
and to the scanning electron microscopy. Cryosectioning was useful when we need a
special sectional plane and when the object is too small for the sectioning with
vibratome.
Our method is useful in the study of any small object with a complex internal
structure although we found it the most useful in our study on the embryonic
development.
ISSN
0582-6802
Language
English
URI
http://hdl.handle.net/10371/7355
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 35 No.4 (1994)
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