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Effect of Kinetin on In Vitro Development of Parthenogenetic Porcine Oocytes Exposed to Demecolcine Prior to Activation

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dc.contributor.authorKim, Kiyoung-
dc.contributor.authorPark, Sang Kyu-
dc.contributor.authorRoh, Sangho-
dc.date.accessioned2011-10-19T08:31:22Z-
dc.date.available2011-10-19T08:31:22Z-
dc.date.issued2009-
dc.identifier.citationJ. Emb. Trans. 24(2):105-108en
dc.identifier.issn1225-4991-
dc.identifier.urihttp://hdl.handle.net/10371/74351-
dc.description.abstractThis study was designed to investigate the effect of kinetin on in vitro development of parthenogenetic porcine oocytes exposed to demecolcine prior to activation. In vitro matured metaphase II stage oocytes were incubated in 0 or 2 g/ml demecolcine supplemented defined culture medium for 3 h and the oocytes were activated electrically. The parthenogenetic porcine embryos were then cultured in 0 or 200 M kinetin supplemented defined culture medium for 7 days. Regardless of demecolcine treatment, kinetin supplementation increased blastocyst rates significantly (7.0% versus 12.1% and 4.9% versus 8.5%; Control versus Kinetin and Demecolcine versus Kinetin + Demecolcine, respectively, p<0.05). Demecolcine treatment before activation tended to decrease blastocyst rates regardless of kinetin supplementation although it is not statistically significant. Total cell numbers in the blastocysts also tended to be elevated in embryos when supplemented with kinetin, however only the result between Kinetin and Demecolcine groups is statistically significant (37.6 7.2 versus 28.1 9.5, respectively, p<0.05). In conclusion, the present report shows that kinetin enhances developmental competence of parthenogenetic porcine embryo regardless of demecolcine pre-treatment before parthenogenetic activation when they were developed in defined culture condition.en
dc.description.sponsorshipThis study was supported by the Korea Science and Engineering Foundation(KOSEF) grant funded by the Ministry of Education Science and Technology(MEST;R01-2008-000-20326-0 and 2008-04347), Republic of Korea.en
dc.language.isoenen
dc.publisherThe Korean Society of Embryo Transferen
dc.subjectkinetinen
dc.subjectdemecolcineen
dc.subjectporcine embryoen
dc.subjectin vitro cultureen
dc.subjectparthenogenesisen
dc.titleEffect of Kinetin on In Vitro Development of Parthenogenetic Porcine Oocytes Exposed to Demecolcine Prior to Activationen
dc.typeArticleen
dc.contributor.AlternativeAuthor김기영-
dc.contributor.AlternativeAuthor박상규-
dc.contributor.AlternativeAuthor노상호-
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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