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The targeting of endothelial progenitor cells to a specific location within a microfluidic channel using magnetic nanoparticles

Cited 43 time in Web of Science Cited 41 time in Scopus
Authors
Kim, Jeong Ah; Lee, Hong Jai; Kang, Hyun-Jae; Park, Tai Hyun
Issue Date
2009-02
Publisher
SPRINGER
Citation
BIOMEDICAL MICRODEVICES; Vol.11 1; 287-296
Keywords
Magnetic nanoparticleEndothelial progenitor cellCirculatory systemMicrofluidic system
Abstract
A common problem with the in vivo therapeutic applications of cells is that cells can rapidly disappear into the circulatory system after an injection. Magnetic nanoparticles can be used to solve this problem. Bacterial magnetic nanoparticles were used in this study for targeting stem cells at a specific location within a microfluidic channel. Magnetic nanoparticles were isolated from Magnetospirillum sp. AMB-1 and delivered to endothelial progenitor cells (EPCs). Cellular uptake of magnetic nanoparticles and their functional feasibility was characterized in vitro. The environment of a human blood vessel was simulated using a microfluidic channel. Magnetic nanoparticle-incorporated EPCs were injected into a microchannel and the flow rate of cells was uniformly controlled by use of a syringe pump. EPCs were effectively targeted to a specific location within the microchannel by an external magnetic field (about 400 mT). About 40% of EPCs were efficiently targeted with a flow rate of 5 mu l min(-1) when 10 mu g of magnetic nanoparticles were used per 10(4) cells. This microfluidic system provides a useful tool towards a better understanding of the behavior of magnetic nanoparticle-incorporated cells within the human circulatory system for clinical use.
ISSN
1387-2176
Language
English
URI
http://hdl.handle.net/10371/76382
DOI
https://doi.org/10.1007/s10544-008-9235-y
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Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
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