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Helicobacter pylori genotyping findings from multiple cultured isolates and mucosal biopsy specimens: strain diversities of Helicobacter pylori isolates in individual hosts
DC Field | Value | Language |
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dc.contributor.author | Kim, Young Sun | - |
dc.contributor.author | Kim, Nayoung | - |
dc.contributor.author | Kim, Jung Mogg | - |
dc.contributor.author | Kim, Mi Soon | - |
dc.contributor.author | Lee, Mi Kyoung | - |
dc.contributor.author | Kim, Joo Sung | - |
dc.contributor.author | Song, In Sung | - |
dc.contributor.author | Jung, Hyun Chae | - |
dc.contributor.author | Lee, Dong Ho | - |
dc.contributor.author | Park, Ji Hyun | - |
dc.date.accessioned | 2012-05-24T07:25:38Z | - |
dc.date.available | 2012-05-24T07:25:38Z | - |
dc.date.issued | 2009-05 | - |
dc.identifier.citation | EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY; Vol.21 5; 522-528 | ko_KR |
dc.identifier.issn | 0954-691X | - |
dc.identifier.uri | https://hdl.handle.net/10371/76440 | - |
dc.description.abstract | Objectives To determine whether the genotypes of virulent genes in Helicobacter pylori isolates and mucosal biopsy specimens differ in individuals, and to investigate whether different isolates from single hosts show strain differences. Methods Sixty-one Korean patients with H. pylori infection were enrolled. PCR and DNA sequencing for cagA, vacA, iceA, and oipA were performed using DNA extracted from H. pylori isolates cultured (2.6 H. pylori isolates per host) directly from antral mucosal biopsy specimens. Strain diversities were analyzed in 234 H. pylori isolates obtained from 43 hosts with at least two H. pylori isolates from antrum and body, respectively, and random amplified polymorphic DNA fingerprinting was carried out on isolates obtained from patients who showed genotype diversity. Results The patients with inconsistent genotyping results between H. pylori isolates and mucosal biopsies were as follows: 16.4% for cagA, 19.7% for vacA m, 47.5% for vacA s1, 6.6% for vacA i-region, 34.4% for iceA, and 21.3% for oipA. Genotyping of H. pylori isolates from same hosts showed diversity in 58.1% (25/43 patients). When random amplified polymorphic DNA-PCR fingerprinting was carried out on 104 H. pylori isolates from 19 patients who showed genotype diversity among their isolates, 68.4% (13 of 19 patients) of patients were found to be colonized by multiple H. pylori strains. Conclusion This study shows that the genotypes of virulent genes from biopsy samples produced different results when compared with those obtained from H. pylori isolates, especially for vacA s1, and iceA. In addition, about 60% of our patients were infected by multiple H. pylori strains. Eur J Gastroenterol Hepatol 21:522-528 (c) 2009 Wolters Kluwer Health | Lippincott Williams & Wilkins. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | LIPPINCOTT WILLIAMS & WILKINS | ko_KR |
dc.subject | diversities | ko_KR |
dc.subject | Helicobacter pylori | ko_KR |
dc.subject | genotype | ko_KR |
dc.title | Helicobacter pylori genotyping findings from multiple cultured isolates and mucosal biopsy specimens: strain diversities of Helicobacter pylori isolates in individual hosts | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 김영순 | - |
dc.contributor.AlternativeAuthor | 김나영 | - |
dc.contributor.AlternativeAuthor | 김정목 | - |
dc.contributor.AlternativeAuthor | 김미순 | - |
dc.contributor.AlternativeAuthor | 박지현 | - |
dc.contributor.AlternativeAuthor | 이미경 | - |
dc.contributor.AlternativeAuthor | 이동호 | - |
dc.contributor.AlternativeAuthor | 김주성 | - |
dc.contributor.AlternativeAuthor | 정현채 | - |
dc.contributor.AlternativeAuthor | 송인성 | - |
dc.identifier.doi | 10.1097/MEG.0b013e3283196af0 | - |
dc.citation.journaltitle | EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY | - |
dc.description.citedreference | Ogiwara H, 2008, GASTROENTEROLOGY, V134, P1267, DOI 10.1053/j.gastro.2007.11.062 | - |
dc.description.citedreference | Rhead JL, 2007, GASTROENTEROLOGY, V133, P926, DOI 10.1053/j.gastro.2007.06.056 | - |
dc.description.citedreference | Yim JY, 2007, HELICOBACTER, V12, P333 | - |
dc.description.citedreference | Salama NR, 2007, J BACTERIOL, V189, P3834, DOI 10.1128/JB.01696-06 | - |
dc.description.citedreference | Erzin Y, 2006, HELICOBACTER, V11, P574 | - |
dc.description.citedreference | Kim N, 2006, HELICOBACTER, V11, P105 | - |
dc.description.citedreference | KIM JW, 2004, KOREAN J INTERN MED, V19, P1 | - |
dc.description.citedreference | Lee JH, 2004, HELICOBACTER, V9, P54 | - |
dc.description.citedreference | Sicinschi LA, 2003, HELICOBACTER, V8, P601 | - |
dc.description.citedreference | Park CY, 2003, J CLIN MICROBIOL, V41, P3336, DOI 10.1128/JCM.41.7.3336-3338.2003 | - |
dc.description.citedreference | Tomasini ML, 2003, J CLIN MICROBIOL, V41, P976, DOI 10.1128/JCM.41.3.976-980.2003 | - |
dc.description.citedreference | Parker J, 2002, BRIT J IND RELAT, V40, P23 | - |
dc.description.citedreference | KIM JL, 2002, KOREAN J GASTROINTES, V25, P7 | - |
dc.description.citedreference | Salama N, 2000, P NATL ACAD SCI USA, V97, P14668, DOI 10.1073/pnas.97.26.14668 | - |
dc.description.citedreference | Yamaoka Y, 2000, P NATL ACAD SCI USA, V97, P7533, DOI 10.1073/pnas.130079797 | - |
dc.description.citedreference | Mukhopadhyay AK, 2000, J BACTERIOL, V182, P3219, DOI 10.1128/JB.182.11.3219-3227.2000 | - |
dc.description.citedreference | van Doorn LJ, 2000, J CLIN MICROBIOL, V38, P13 | - |
dc.description.citedreference | Yamaoka Y, 1999, J CLIN MICROBIOL, V37, P2274 | - |
dc.description.citedreference | van Doorn LJ, 1999, GASTROENTEROLOGY, V116, P823 | - |
dc.description.citedreference | Lu JJ, 1999, J CLIN MICROBIOL, V37, P772 | - |
dc.description.citedreference | Yamaoka Y, 1998, HELICOBACTER, V3, P241 | - |
dc.description.citedreference | Wang HJ, 1998, BIOCHEM BIOPH RES CO, V250, P397 | - |
dc.description.citedreference | van Doorn LJ, 1998, GASTROENTEROLOGY, V115, P58 | - |
dc.description.citedreference | *KOR H PYL STUD GR, 1998, J KOREAN GASTROENTER, V32, P275 | - |
dc.description.citedreference | Atherton JC, 1997, GUT, V40, P701 | - |
dc.description.citedreference | ATHERTON JC, 1995, J BIOL CHEM, V270, P17771 | - |
dc.description.citedreference | BLASER MJ, 1995, CANCER RES, V55, P2111 | - |
dc.description.citedreference | CLAYTON CL, 1992, J CLIN MICROBIOL, V30, P192 | - |
dc.description.citedreference | HO SA, 1991, J CLIN MICROBIOL, V29, P2543 | - |
dc.description.citedreference | WANG Y, 1990, J BACTERIOL, V172, P949 | - |
dc.description.citedreference | KWOK S, 1989, NATURE, V339, P237, DOI 10.1038/339237a0 | - |
dc.description.tc | 7 | - |
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