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Wnt inhibitory factor (WIF)-1 inhibits osteoblastic differentiation in mouse embryonic mesenchymal cells
DC Field | Value | Language |
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dc.contributor.author | Cho, Sun Wook | - |
dc.contributor.author | Yang, Jae-Yeon | - |
dc.contributor.author | Sun, Hyun Jin | - |
dc.contributor.author | Jung, Ju Yeon | - |
dc.contributor.author | Cho, Hwa Young | - |
dc.contributor.author | Kim, Sang Wan | - |
dc.contributor.author | Shin, Chan Soo | - |
dc.contributor.author | Kim, Seong Yeon | - |
dc.contributor.author | Choi, Hyung Jin | - |
dc.contributor.author | Her, Sun Ju | - |
dc.date.accessioned | 2012-05-25T00:51:14Z | - |
dc.date.available | 2012-05-25T00:51:14Z | - |
dc.date.issued | 2009-06 | - |
dc.identifier.citation | BONE; Vol.44 6; 1069-1077 | ko_KR |
dc.identifier.issn | 8756-3282 | - |
dc.identifier.uri | https://hdl.handle.net/10371/76446 | - |
dc.description.abstract | Wnt inhibitory factor (WIF)-1 belongs to the members of secreted modulators of Wnt proteins. Secreted frizzled-related proteins (sFRPs), another member of Wnt modulators, have been shown to play differential roles in Writ signaling depending on the subtypes and cell models. This Study was undertaken to investigate the functional role of WIF-1 in osteoblastic differentiation of mouse mesenchymal C3H10T1/2 cells. C3H10T1/2 cells express endogenous WIF-1 and its expression level decreases during osteoblastogenesis. Treatment of C3H10T1/2 cells with WIF-1 significantly reduced alkaline phosphatase (ALP) activities induced by either osteogenic medium (OM, ascorbic acid and beta-glycerophosphate) or Wnt-3a conditioned medium (CM) in a dose-dependent manner. In contrast, the expression level of endogenous WIF-1 increased during adipogenesis and WIF-1 treatment resulted in increased adipogenesis. C3H10T1/2 cells transduced with WIF-1 retrovirus also exhibited reduced ALP activity and decreased mRNA expression of Runx2, collagen type 1, ALP and cisteocalcin during osteoblastic differentiation compared to empty virus-transduced cells. Moreover, treatment with WIF-1 dose-dependently attenuates beta-catenin/T-cell factor (TCF) transcriptional activity in this cell line. Finally, knockdown of WIF-1 in C3H10T1/2 cells by RNA interference leads to increase in ALP activities. Collectively, these results indicate that WIF-1 plays as a negative regulator of osteoblastic differentiation in mouse mesenchymal C3H10T1/2 cells in vitro. (C) 2009 Elsevier Inc. All rights reserved. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | ELSEVIER SCIENCE INC | ko_KR |
dc.subject | Wnt inhibitory factor-1 | ko_KR |
dc.subject | Adipogenesis | ko_KR |
dc.subject | Mesenchymal cell | ko_KR |
dc.subject | Osteoblastic differentiation | ko_KR |
dc.subject | Wnt | ko_KR |
dc.title | Wnt inhibitory factor (WIF)-1 inhibits osteoblastic differentiation in mouse embryonic mesenchymal cells | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 조선욱 | - |
dc.contributor.AlternativeAuthor | 양재연 | - |
dc.contributor.AlternativeAuthor | 선현진 | - |
dc.contributor.AlternativeAuthor | 정주연 | - |
dc.contributor.AlternativeAuthor | 허선주 | - |
dc.contributor.AlternativeAuthor | 조화영 | - |
dc.contributor.AlternativeAuthor | 최형진 | - |
dc.contributor.AlternativeAuthor | 김상완 | - |
dc.contributor.AlternativeAuthor | 김성연 | - |
dc.contributor.AlternativeAuthor | 신찬수 | - |
dc.identifier.doi | 10.1016/j.bone.2009.02.012 | - |
dc.citation.journaltitle | BONE | - |
dc.description.citedreference | Cho SW, 2008, BIOCHEM BIOPH RES CO, V367, P399, DOI 10.1016/j.bbrc.2007.12.128 | - |
dc.description.citedreference | Li X, 2007, J BONE MINER RES, V22, pS65 | - |
dc.description.citedreference | Yaccoby S, 2007, BLOOD, V109, P2106, DOI 10.1182/blood-2006-09-047712 | - |
dc.description.citedreference | Bodine PVN, 2006, REV ENDOCR METAB DIS, V7, P33, DOI 10.1007/s11154-006-9002-4 | - |
dc.description.citedreference | Yang LJ, 2006, J DERMATOL SCI, V42, P111, DOI 10.1016/j.jdermsci.2005.12.011 | - |
dc.description.citedreference | Galli LM, 2006, DEV DYNAM, V235, P681, DOI 10.1002/dvdy.20681 | - |
dc.description.citedreference | Nakashima A, 2005, J BIOL CHEM, V280, P37660, DOI 10.1074/jbc.M504612200 | - |
dc.description.citedreference | van der Horst G, 2005, J BONE MINER RES, V20, P1867, DOI 10.1359/JBMR.050614 | - |
dc.description.citedreference | Holmen SL, 2004, J BONE MINER RES, V19, P2033, DOI 10.1359/JBMR.040907 | - |
dc.description.citedreference | Reguart N, 2004, BIOCHEM BIOPH RES CO, V323, P229, DOI 10.1016/j.bbrc.2004.08.075 | - |
dc.description.citedreference | Chung YS, 2004, J BONE MINER RES, V19, P1395, DOI 10.1359/JBMR.040412 | - |
dc.description.citedreference | Nuttall ME, 2004, CURR OPIN PHARMACOL, V4, P290, DOI 10.1016/j.coph.2004.03.002 | - |
dc.description.citedreference | He X, 2004, DEVELOPMENT, V131, P1663, DOI 10.1242/dev.01117 | - |
dc.description.citedreference | Rawadi G, 2003, J BONE MINER RES, V18, P1842 | - |
dc.description.citedreference | Babij P, 2003, J BONE MINER RES, V18, P960 | - |
dc.description.citedreference | Vaes BLT, 2002, J BONE MINER RES, V17, P2106 | - |
dc.description.citedreference | Bennett CN, 2002, J BIOL CHEM, V277, P30998, DOI 10.1074/jbc.M204527200 | - |
dc.description.citedreference | Mao BY, 2002, NATURE, V417, P664 | - |
dc.description.citedreference | Little RD, 2002, AM J HUM GENET, V70, P11 | - |
dc.description.citedreference | Gong YQ, 2001, CELL, V107, P513 | - |
dc.description.citedreference | Furlan F, 2001, CELL COMMUN ADHES, V8, P367 | - |
dc.description.citedreference | Ellies DL, 2000, DEVELOPMENT, V127, P5285 | - |
dc.description.citedreference | Hsieh JC, 1999, NATURE, V398, P431 | - |
dc.description.citedreference | Polakis P, 1999, CURR OPIN GENET DEV, V9, P15 | - |
dc.description.citedreference | Shibamoto S, 1998, GENES CELLS, V3, P659 | - |
dc.description.citedreference | Cadigan KM, 1997, GENE DEV, V11, P3286 | - |
dc.description.citedreference | Melkonyan HS, 1997, P NATL ACAD SCI USA, V94, P13636 | - |
dc.description.citedreference | Shimizu H, 1997, CELL GROWTH DIFFER, V8, P1349 | - |
dc.description.citedreference | Wang SW, 1997, CELL, V88, P757 | - |
dc.description.citedreference | NUSSE R, 1982, CELL, V31, P99 | - |
dc.description.tc | 11 | - |
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