Publications
Detailed Information
Activation of AMP-activated protein kinase stimulates the nuclear localization of glyceraldehyde 3-phosphate dehydrogenase in human diploid fibroblasts
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kwon, Hyun Jin | - |
dc.contributor.author | Rhim, Ji Heon | - |
dc.contributor.author | Jang, Il-Soon | - |
dc.contributor.author | Kim, Go-Eun | - |
dc.contributor.author | Yeo, Eui-Ju | - |
dc.contributor.author | Park, Sang Chul | - |
dc.date.accessioned | 2012-06-05T05:41:10Z | - |
dc.date.available | 2012-06-05T05:41:10Z | - |
dc.date.issued | 2010-04-30 | - |
dc.identifier.citation | EXPERIMENTAL AND MOLECULAR MEDICINE; Vol.42 4; 254-269 | ko_KR |
dc.identifier.issn | 1226-3613 | - |
dc.identifier.uri | https://hdl.handle.net/10371/76848 | - |
dc.description.abstract | In addition to its well-known glycolytic activity, GAPDH displays multiple functions, such as nuclear RNA export, DNA replication and repair, and apoptotic cell death. This functional diversity depends on its intracellular localization. In this study, we explored the signal transduction pathways involved in the nuclear translocation of GAPDH using confocal laser scanning microscopy of immunostained human diploid fibroblasts (HDFs). GAPDH was present mainly in the cytoplasm when cultured with 10% FBS. Serum depletion by culturing cells in a serum-free medium (SFM) led to a gradual accumulation of GAPDH in the nucleus, and this nuclear accumulation was reversed by the re-addition of serum or growth factors, such as PDGF and lysophosphatidic acid. The nuclear export induced by the re-addition of serum or growth factors was prevented by LY 294002 and SH-5, inhibitors of phosphoinositide 3-kinase (PI3K) and Akt/protein kinase B, respectively, suggesting an involvement of the PI3K signaling pathway in the nuclear export of GAPDH. In addition, 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (AICAR), an activator of AMP-activated protein kinase (AMPK), stimulated the nuclear translocation of GAPDH and prevented serum- and growth factor-induced GAPDH export. AMPK inhibition by compound C or AMPK depletion by siRNA treatment partially prevented SFM- and AICAR-induced nuclear translocation of GAPDH. Our data suggest that the nuclear translocation of GAPDH might be regulated by the PI3K signaling pathway acting mainly as a nuclear export signal and the AMPK signaling pathway acting as a nuclear import signal. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | KOREAN SOC MED BIOCHEMISTRY MOLECULAR BIOLOGY | ko_KR |
dc.subject | AICA ribonucleotide | ko_KR |
dc.subject | 1-phosphatidylinositol 3-kinase | ko_KR |
dc.subject | glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) | ko_KR |
dc.subject | AMP-actived protein kinases | ko_KR |
dc.subject | fibroblasts | ko_KR |
dc.title | Activation of AMP-activated protein kinase stimulates the nuclear localization of glyceraldehyde 3-phosphate dehydrogenase in human diploid fibroblasts | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 권현진 | - |
dc.contributor.AlternativeAuthor | 임지헌 | - |
dc.contributor.AlternativeAuthor | 장일순 | - |
dc.contributor.AlternativeAuthor | 김고은 | - |
dc.contributor.AlternativeAuthor | 박상철 | - |
dc.contributor.AlternativeAuthor | 여의주 | - |
dc.identifier.doi | 10.3858/emm.2010.42.4.025 | - |
dc.citation.journaltitle | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.description.citedreference | Peairs A, 2009, CLIN EXP IMMUNOL, V156, P542, DOI 10.1111/j.1365-2249.2009.03924.x | - |
dc.description.citedreference | Chen Z, 2009, CIRC RES, V104, P496, DOI 10.1161/CIRCRESAHA.108.187567 | - |
dc.description.citedreference | Cao C, 2008, J BIOL CHEM, V283, P28897, DOI 10.1074/jbc.M804144200 | - |
dc.description.citedreference | Li XX, 2008, ARTERIOSCL THROM VAS, V28, P1789, DOI 10.1161/ATVBAHA.108.172452 | - |
dc.description.citedreference | Lombardi M, 2008, J CELL BIOL, V182, P327 | - |
dc.description.citedreference | Sen N, 2008, NAT CELL BIOL, V10, P866, DOI 10.1038/ncb1747 | - |
dc.description.citedreference | Kim HS, 2008, J BIOL CHEM, V283, P3731, DOI 10.1074/jbc.M704432200 | - |
dc.description.citedreference | Du ZX, 2007, ENDOCRINOLOGY, V148, P4352, DOI 10.1210/en.2006-1511 | - |
dc.description.citedreference | Harada N, 2007, J BIOL CHEM, V282, P22651, DOI 10.1074/jbc.M610724200 | - |
dc.description.citedreference | Goirand F, 2007, J PHYSIOL-LONDON, V581, P1163, DOI 10.1113/jphysiol.2007.132589 | - |
dc.description.citedreference | Barbini L, 2007, MOL CELL BIOCHEM, V300, P19, DOI 10.1007/s11010-006-9341-1 | - |
dc.description.citedreference | Hurley RL, 2006, J BIOL CHEM, V281, P36662, DOI 10.1074/jbc.M606676200 | - |
dc.description.citedreference | Hara MR, 2006, CELL MOL NEUROBIOL, V26, P527, DOI 10.1007/s10571-006-9011-6 | - |
dc.description.citedreference | Tisdale EJ, 2006, J BIOL CHEM, V281, P8436, DOI 10.1074/jbc.M513031200 | - |
dc.description.citedreference | Rattan R, 2005, J BIOL CHEM, V280, P39582, DOI 10.1074/jbc.M507443200 | - |
dc.description.citedreference | Hara MR, 2005, NAT CELL BIOL, V7, P665, DOI 10.1038/ncb1268 | - |
dc.description.citedreference | Sirover MA, 2005, J CELL BIOCHEM, V95, P45, DOI 10.1002/jcb.20399 | - |
dc.description.citedreference | Jones RG, 2005, MOL CELL, V18, P283, DOI 10.1016/j.molcel.2005.03.027 | - |
dc.description.citedreference | Tisdale EJ, 2004, J BIOL CHEM, V279, P54046, DOI 10.1074/jbc.M409472200 | - |
dc.description.citedreference | Hardie DG, 2004, J CELL SCI, V117, P5479, DOI 10.1242/jcs.01540 | - |
dc.description.citedreference | Li J, 2004, AM J PHYSIOL-ENDOC M, V287, pE834, DOI 10.1152/ajpendo.00234.2004 | - |
dc.description.citedreference | Cooray S, 2004, J GEN VIROL, V85, P1065, DOI 10.1099/vir.0.1977-0 | - |
dc.description.citedreference | Brown VM, 2004, J BIOL CHEM, V279, P5984, DOI 10.1074/jbc.M307071200 | - |
dc.description.citedreference | Tisdale EJ, 2003, J BIOL CHEM, V278, P52524, DOI 10.1074/jbc.M309343200 | - |
dc.description.citedreference | HAWLEY SA, 2003, J BIOL, V2, P28 | - |
dc.description.citedreference | Schmitz HD, 2003, CELL BIOL INT, V27, P511, DOI 10.1011/S1065-6995(03)00096-9 | - |
dc.description.citedreference | Tisdale EJ, 2002, J BIOL CHEM, V277, P3334, DOI 10.1074/jbc.M109744200 | - |
dc.description.citedreference | Schmitz HD, 2001, EUR J CELL BIOL, V80, P419 | - |
dc.description.citedreference | Dastoor Z, 2001, J CELL SCI, V114, P1643 | - |
dc.description.citedreference | Yeo EJ, 2000, MOL CELLS, V10, P415 | - |
dc.description.citedreference | Stein SC, 2000, BIOCHEM J, V345, P437 | - |
dc.description.citedreference | Sirover MA, 1999, BBA-PROTEIN STRUCT M, V1432, P159 | - |
dc.description.citedreference | Shashidharan P, 1999, NEUROREPORT, V10, P1149 | - |
dc.description.citedreference | Rameh LE, 1999, J BIOL CHEM, V274, P8347 | - |
dc.description.citedreference | Sawa A, 1997, P NATL ACAD SCI USA, V94, P11669 | - |
dc.description.citedreference | Vincent MF, 1996, BIOCHEM PHARMACOL, V52, P999 | - |
dc.description.citedreference | Reiss N, 1996, BIOCHEM MOL BIOL INT, V38, P711 | - |
dc.description.citedreference | CORTON JM, 1995, EUR J BIOCHEM, V229, P558 | - |
dc.description.citedreference | KAWAMOTO RM, 1986, BIOCHEMISTRY-US, V25, P657 | - |
dc.description.citedreference | BOYCE ST, 1983, J INVEST DERMATOL S, V81, P33 | - |
dc.description.tc | 4 | - |
- Appears in Collections:
- Files in This Item:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.