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Stimulatory heterotrimeric GTP-binding protein augments cisplatin-induced apoptosis by upregulating Bak expression in human lung cancer cells
DC Field | Value | Language |
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dc.contributor.author | Choi, Yoon Jung | - |
dc.contributor.author | Oh, Jung-Min | - |
dc.contributor.author | Kim, So-Young | - |
dc.contributor.author | Seo, Miran | - |
dc.contributor.author | Juhnn, Yong-Sung | - |
dc.date.accessioned | 2012-06-05T06:07:32Z | - |
dc.date.available | 2012-06-05T06:07:32Z | - |
dc.date.issued | 2009-06 | - |
dc.identifier.citation | CANCER SCIENCE; Vol.100 6; 1069-1074 | ko_KR |
dc.identifier.issn | 1347-9032 | - |
dc.identifier.uri | https://hdl.handle.net/10371/76854 | - |
dc.description.abstract | The present study aimed to investigate the effect of the stimulatory heterotrimeric GTP-binding (Gs) protein signaling system on cisplatin-induced apoptosis of lung cancer cells and its underlying mechanism as an attempt to develop a novel strategy to improve the therapeutic efficacy of cisplatin. Overexpression of the constitutively active alpha subunit of Gs (G alpha sQL) in A549 human lung cancer cells increased cisplatin-induced apoptosis, and knockdown of G alpha s with small hairpin RNA decreased the percentage of apoptotic cells. G alpha sQL increased the expression of the proapoptotic proteins B-cell leukemia/lymphoma-2 genes (Bcl-2) homologous antagonist killer protein (Bak) and Bcl-2 associated X protein (Bax), and decreased the expression of the antiapoptotic proteins Bcl-2 and Bcl-Xlong protein. Knockdown of Bak blocked the augmentative effects of G alpha sQL. G alpha sQL decreased the degradation rate of the Bak protein, and increased Bak mRNA transcript levels. G alpha sQL increased Bak-luciferase activity in a protein kinase A and cyclic AMP response element-dependent manner. G alpha sQL also augmented cisplatin-induced apoptosis of H1299 human lung cancer cells that lack functional p53. From this study, it is concluded that G alpha s augments cisplatin-induced apoptosis of lung cancer cells partially through upregulating Bak expression by increasing transcription and by decreasing the rate of protein degradation. (Cancer Sci 2009; 100: 1069-1074). | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | WILEY-BLACKWELL PUBLISHING, INC | ko_KR |
dc.title | Stimulatory heterotrimeric GTP-binding protein augments cisplatin-induced apoptosis by upregulating Bak expression in human lung cancer cells | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 최윤정 | - |
dc.contributor.AlternativeAuthor | 오정민 | - |
dc.contributor.AlternativeAuthor | 김소영 | - |
dc.contributor.AlternativeAuthor | 서미란 | - |
dc.contributor.AlternativeAuthor | 전용성 | - |
dc.identifier.doi | 10.1111/j.1349-7006.2009.01136.x | - |
dc.citation.journaltitle | CANCER SCIENCE | - |
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dc.description.tc | 1 | - |
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