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Complement depletion with cobra venom factor delays acute cell-mediated rejection in pig-to-mouse corneal xenotransplantation

Cited 14 time in Web of Science Cited 14 time in Scopus
Authors
Oh, Joo Youn; Kim, Mee Kum; Lee, Hyun Ju; Ko, Jung Hwa; Park, Chan-Sik; Park, Chung-Gyu; Lee, Jin Hak; Wee, Won Ryang; Kim, Sang Joon; Kang, Hee Jung; Kim, Youngji
Issue Date
2010-04
Publisher
WILEY-BLACKWELL
Citation
XENOTRANSPLANTATION; Vol.17 2; 140-146
Keywords
cobra venom factorcomplementpigmousecorneaxenotransplantation
Abstract
Background: We have demonstrated earlier that porcine corneal xenografts underwent an acute cell-mediated rejection in mice despite the absence of T cells. In the present study, we investigated the effect of complement depletion by cobra venom factor (CVF) on the corneal xenograft rejection in a pig-to-mouse model. Methods: Porcine corneas were orthotopically transplanted into C57BL/6 (B6) and severe combined immunodeficiency (SCID) mice. For complement depletion, 25 mu g of CVF (1 g/kg bodyweight) was injected intraperitoneally on the day before and 1, 3, 5, and 7 days after transplantation. Graft survival was clinically assessed by slit lamp biomicroscopy and the median survival time (MST) was calculated. The grafts were histologically evaluated serially after transplantation using antibodies against CD4, CD8, NK1.1, and F4/80. Results: The CVF treatment significantly prolonged the porcine corneal xenograft survival in both B6 (MST 9.4 vs. 15.5 days; P = 0.0011) and SCID mice (MST 16.4 vs. 20.5 days; P = 0.0474). Histologically, whereas macrophages and CD4(+) T cells were progressively in. ltrated into porcine corneal grafts in CVF-untreated B6 mice, the infiltration by both cells was markedly delayed and decreased in the xenografts in CVF-treated B6 mice. Likewise, macrophage infiltration, which was prominent in rejected porcine xenografts in SCID mice, was also reduced in CVF-treated SCID mice. Conclusions: Our results suggest that complement depletion by CVF delayed, although did not prevent, an acute cell-mediated rejection in a pig-to-mouse corneal xenotransplantation.
ISSN
0908-665X
Language
English
URI
http://hdl.handle.net/10371/76936
DOI
https://doi.org/10.1111/j.1399-3089.2010.00574.x
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College of Medicine/School of Medicine (의과대학/대학원)Microbiology (미생물학전공)Journal Papers (저널논문_미생물학전공)
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