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Mass spectrometric analysis of the glycosphingolipid-derived glycans from miniature pig endothelial cells and islets: identification of NeuGc epitope in pig islets

Cited 10 time in Web of Science Cited 10 time in Scopus
Authors
Kim, Yun-Gon; Harvey, David J.; Yang, Yung-Hun; Park, Chung-Gyu; Kim, Byung-Gee
Issue Date
2009-10
Publisher
JOHN WILEY & SONS LTD
Citation
JOURNAL OF MASS SPECTROMETRY; Vol.44 10; 1489-1499
Keywords
xenotransplantationmass spectrometryN-glycolylneuraminic acidpig islet cellspig endothelial cellsglycosphingolipid
Abstract
Glycosphingolipid (GSL) is a major component of the plasma membrane in eukaryotic cells that is involved directly in a variety of immunological events via cell-to-cell or cell-to-protein interactions. In this study, qualitative and quantitative analyses of GSL-derived glycans on endothelial cells and islets from a miniature pig were performed and their glycosylation patterns were compared. A total of 60 and 47 sialylated and neutral GSL-derived glycans from the endothelial cells and islets, respectively, were characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and collision-induced fragmentation using positive-ion electrospray ionization (ESI) ion-trap tandem mass spectrometry (MS/MS). In accordance with previous immunohistochemistry studies, the alpha-Gal-terminated GSL was not detected but NeuGc-terminated GSLs were newly detected from miniature pig islets. In addition, the neutral GSL-derived glycans were relatively quantified by derivatization with carboxymethyl trimethylammonium hydrazide (so called Girard`s T reagent) and MALDI-TOF MS. The structural information of the GSL-derived glycans from pig endothelial cells and islets suggests that special attention should be paid to all types of glycoconjugates expressed on pig tissues or cells for successful clinical xenotransplantation. Copyright (C) 2009 John Wiley & Sons, Ltd.
ISSN
1076-5174
Language
English
URI
http://hdl.handle.net/10371/76940
DOI
https://doi.org/10.1002/jms.1638
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College of Medicine/School of Medicine (의과대학/대학원)Microbiology (미생물학전공)Journal Papers (저널논문_미생물학전공)
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