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Qualitative and quantitative comparison of N-glycans between pig endothelial and islet cells by high-performance liquid chromatography and mass spectrometry-based strategy

Cited 22 time in Web of Science Cited 22 time in Scopus
Authors
Kim, Yun-Gon; Gil, Geun-Cheol; Jang, Kyung-Soon; Lee, Sukmook; Kim, Jung-Sik; Park, Chung-Gyu; Kim, Byung-Gee; Harvey, David J.; Chung, Junho; Kim, Hyoung-Il
Issue Date
2009-07
Publisher
JOHN WILEY & SONS LTD
Citation
JOURNAL OF MASS SPECTROMETRY; Vol.44 7; 1087-1104
Keywords
xenotransplantationpig islet cellspig endothelial cellN-glycolyl-neuraminic acidalpha-galactosemass spectrometry
Abstract
N-glycan structures released from miniature pig endothelial and islet cells were determined by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) negative ion electrospray ionization (ESI) MS/MS and normal-phase high performance liquid chromatography (NP-HPLC) combined with exoglycosidase digestion. Totally, the identified structures were 181 N-glycans including 129 sialylated and 18 alpha-galactosylated glycans from pig endothelial cells and 80 N-glycans including 41 sialylated and one alpha-galactosylated glycans from pig islet cells. The quantity of the alpha-galactosylated glycans from pig islet cells was certainly neglectable compared to pig endothelial cells. A number of NeuGc-terminated N-glycans (80 from pig endothelial cells and 13 from pig islet cells) are newly detected by our mass spectrometric strategies. The detailed structural information will be a matter of great interest in organ or cell xenotransplantation using alpha 1,3-galactosyltransferase gene-knockout (GaIT-KO) pig. Copyright (C) 2009 John Wiley & Sons, Ltd.
ISSN
1076-5174
Language
English
URI
http://hdl.handle.net/10371/76947
DOI
https://doi.org/10.1002/jms.1587
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College of Medicine/School of Medicine (의과대학/대학원)Microbiology (미생물학전공)Journal Papers (저널논문_미생물학전공)
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