S-Space College of Medicine/School of Medicine (의과대학/대학원) Microbiology (미생물학전공) Journal Papers (저널논문_미생물학전공)
Qualitative and quantitative comparison of N-glycans between pig endothelial and islet cells by high-performance liquid chromatography and mass spectrometry-based strategy
- Kim, Yun-Gon; Gil, Geun-Cheol; Jang, Kyung-Soon; Lee, Sukmook; Kim, Jung-Sik; Park, Chung-Gyu; Kim, Byung-Gee; Harvey, David J.; Chung, Junho; Kim, Hyoung-Il
- Issue Date
- JOHN WILEY & SONS LTD
- JOURNAL OF MASS SPECTROMETRY; Vol.44 7; 1087-1104
- xenotransplantation; pig islet cells; pig endothelial cell; N-glycolyl-neuraminic acid; alpha-galactose; mass spectrometry
- N-glycan structures released from miniature pig endothelial and islet cells were determined by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) negative ion electrospray ionization (ESI) MS/MS and normal-phase high performance liquid chromatography (NP-HPLC) combined with exoglycosidase digestion. Totally, the identified structures were 181 N-glycans including 129 sialylated and 18 alpha-galactosylated glycans from pig endothelial cells and 80 N-glycans including 41 sialylated and one alpha-galactosylated glycans from pig islet cells. The quantity of the alpha-galactosylated glycans from pig islet cells was certainly neglectable compared to pig endothelial cells. A number of NeuGc-terminated N-glycans (80 from pig endothelial cells and 13 from pig islet cells) are newly detected by our mass spectrometric strategies. The detailed structural information will be a matter of great interest in organ or cell xenotransplantation using alpha 1,3-galactosyltransferase gene-knockout (GaIT-KO) pig. Copyright (C) 2009 John Wiley & Sons, Ltd.
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