Publications
Detailed Information
Control of Adipogenesis by the SUMO-Specific Protease SENP2
Cited 52 time in
Web of Science
Cited 57 time in Scopus
- Authors
- Issue Date
- 2010-05-01
- Publisher
- AMER SOC MICROBIOLOGY
- Citation
- MOLECULAR AND CELLULAR BIOLOGY; Vol.30 9; 2135-2146
- Abstract
- Here, we demonstrate that SENP2, a desumoylating enzyme, plays a critical role in the control of adipogenesis. SENP2 expression was markedly increased upon the induction of adipocyte differentiation, and this increase was dependent on protein kinase A activation. Remarkably, knockdown of SENP2 led to a dramatic attenuation of adipogenesis with a marked decrease in PPAR gamma and C/EBP alpha mRNA levels. Knockdown of SENP2 also caused a marked reduction in the level of C/EBP beta protein but not in that of C/EBP beta mRNA. Interestingly, sumoylation of C/EBP beta dramatically increased its ubiquitination and destabilization, and this increase could be reversed by SENP2. In addition, overexpression of C/EBP beta could overcome the inhibitory effect of SENP2 knockdown on adipogenesis. Furthermore, SENP2 was absolutely required for adipogenesis of preadipocytes implanted into mice. These results establish a critical role for SENP2 in the regulation of adipogenesis by desumoylation and stabilization of C/EBP beta and in turn by promoting the expression of its downstream effectors, such as PPAR gamma and C/EBP alpha.
- ISSN
- 0270-7306
- Language
- English
- Files in This Item:
- There are no files associated with this item.
- Appears in Collections:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.