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Control of Adipogenesis by the SUMO-Specific Protease SENP2

Cited 41 time in Web of Science Cited 42 time in Scopus
Authors
Chung, Sung Soo; Ahn, Byung Yong; Kim, Min; Choi, Hye Hun; Kang, Shinae; Kim, Young-Bum; Lee, Hong Kyu; Park, Kyong Soo; Chung, Chin Ha; Cho, Young Min; Park, Sang Gyu; Park, Ho Seon
Issue Date
2010-05-01
Publisher
AMER SOC MICROBIOLOGY
Citation
MOLECULAR AND CELLULAR BIOLOGY; Vol.30 9; 2135-2146
Abstract
Here, we demonstrate that SENP2, a desumoylating enzyme, plays a critical role in the control of adipogenesis. SENP2 expression was markedly increased upon the induction of adipocyte differentiation, and this increase was dependent on protein kinase A activation. Remarkably, knockdown of SENP2 led to a dramatic attenuation of adipogenesis with a marked decrease in PPAR gamma and C/EBP alpha mRNA levels. Knockdown of SENP2 also caused a marked reduction in the level of C/EBP beta protein but not in that of C/EBP beta mRNA. Interestingly, sumoylation of C/EBP beta dramatically increased its ubiquitination and destabilization, and this increase could be reversed by SENP2. In addition, overexpression of C/EBP beta could overcome the inhibitory effect of SENP2 knockdown on adipogenesis. Furthermore, SENP2 was absolutely required for adipogenesis of preadipocytes implanted into mice. These results establish a critical role for SENP2 in the regulation of adipogenesis by desumoylation and stabilization of C/EBP beta and in turn by promoting the expression of its downstream effectors, such as PPAR gamma and C/EBP alpha.
ISSN
0270-7306
Language
English
URI
http://hdl.handle.net/10371/77430
DOI
https://doi.org/10.1128/MCB.00852-09
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College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
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