S-Space College of Medicine/School of Medicine (의과대학/대학원) Internal Medicine (내과학전공) Journal Papers (저널논문_내과학전공)
Control of Adipogenesis by the SUMO-Specific Protease SENP2
- Chung, Sung Soo; Ahn, Byung Yong; Kim, Min; Choi, Hye Hun; Kang, Shinae; Kim, Young-Bum; Lee, Hong Kyu; Park, Kyong Soo; Chung, Chin Ha; Cho, Young Min; Park, Sang Gyu; Park, Ho Seon
- Issue Date
- AMER SOC MICROBIOLOGY
- MOLECULAR AND CELLULAR BIOLOGY; Vol.30 9; 2135-2146
- Here, we demonstrate that SENP2, a desumoylating enzyme, plays a critical role in the control of adipogenesis. SENP2 expression was markedly increased upon the induction of adipocyte differentiation, and this increase was dependent on protein kinase A activation. Remarkably, knockdown of SENP2 led to a dramatic attenuation of adipogenesis with a marked decrease in PPAR gamma and C/EBP alpha mRNA levels. Knockdown of SENP2 also caused a marked reduction in the level of C/EBP beta protein but not in that of C/EBP beta mRNA. Interestingly, sumoylation of C/EBP beta dramatically increased its ubiquitination and destabilization, and this increase could be reversed by SENP2. In addition, overexpression of C/EBP beta could overcome the inhibitory effect of SENP2 knockdown on adipogenesis. Furthermore, SENP2 was absolutely required for adipogenesis of preadipocytes implanted into mice. These results establish a critical role for SENP2 in the regulation of adipogenesis by desumoylation and stabilization of C/EBP beta and in turn by promoting the expression of its downstream effectors, such as PPAR gamma and C/EBP alpha.
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