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Proteomic Analysis of Toxoplasma gondii KI-1 Tachyzoites
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Si-Hwan | - |
dc.contributor.author | Kim, Tae Yun | - |
dc.contributor.author | Cha, Guang-Ho | - |
dc.contributor.author | Park, Sung Goo | - |
dc.contributor.author | Shin, Dae-Whan | - |
dc.contributor.author | Lee, Young-Ha | - |
dc.contributor.author | Chai, Jong-Yil | - |
dc.date.accessioned | 2012-06-27T04:40:46Z | - |
dc.date.available | 2012-06-27T04:40:46Z | - |
dc.date.issued | 2010-09 | - |
dc.identifier.citation | KOREAN JOURNAL OF PARASITOLOGY; Vol.48 3; 195-201 | ko_KR |
dc.identifier.issn | 0023-4001 | - |
dc.identifier.uri | https://hdl.handle.net/10371/77552 | - |
dc.description.abstract | We studied on the proteomic characteristics of Toxoplasma gondii KI-1 tachyzoites which were originally isolated from a Korean patient, and compared with those of the well-known virulent RH strain using 2-dimensional electrophoresis (2-DE), mass spectrometry, and quantitative real-time PCR. Two-dimensional separation of the total proteins isolated from KI-1 tachyzoites revealed up to 150 spots, of which 121 were consistent with those of RH tachyzoites. Of the remaining 29 spots, 14 showed greater than 5-fold difference in density between the KI-1 and RH tachyzoites at a pH of 5.0-8.0. Among the 14 spots, 5 from the KI-1 isolate and 7 from the RH strain were identified using MALDI-TOF mass spectrometry and database searches. The spots from the KI-1 tachyzoites were dense granule proteins (GRA 2, 3, 6, and 7), hypoxanthine-guanine-xanthine phosphoribosyltransferase (HGRPTase), and uracil phosphoribosyltransferase (UPRTase). The spots from the RH strain were surface antigen 1 (SAG 1), L-lactate dehydrogenase (LDH), actin, chorismate synthase, peroximal catalase, hexokinase, bifunctional dihydrofolate reductase-thymidylate synthase (DHTR-TS), and nucleoside-triphosphatases (NTPases). Quantitative real-time PCR supported our mass spectrometric results by showing the elevated expression of the genes encoding GRA 2, 3, and 6 and UPRTase in the KI-1 tachyzoites and those encoding GRA 7, SAG 1, NTPase, and chorismate synthase in the RH tachyzoites. These observations demonstrate that the protein compositions of KI-1 and RH tachyzoites are similar but differential protein expression is involved in virulence. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | KOREAN SOC PARASITOLOGY, SEOUL NATL UNIV COLL MEDI | ko_KR |
dc.subject | Toxoplasma gondii | ko_KR |
dc.subject | Korean Isolate-1 (KI-1) | ko_KR |
dc.subject | proteomics | ko_KR |
dc.subject | quantitative real time PCR | ko_KR |
dc.title | Proteomic Analysis of Toxoplasma gondii KI-1 Tachyzoites | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 최시환 | - |
dc.contributor.AlternativeAuthor | 김태윤 | - |
dc.contributor.AlternativeAuthor | 박성구 | - |
dc.contributor.AlternativeAuthor | 차광호 | - |
dc.contributor.AlternativeAuthor | 신대환 | - |
dc.contributor.AlternativeAuthor | 채종일 | - |
dc.contributor.AlternativeAuthor | 이영하 | - |
dc.identifier.doi | 10.3347/kjp.2010.48.3.195 | - |
dc.citation.journaltitle | KOREAN JOURNAL OF PARASITOLOGY | - |
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dc.description.tc | 1 | - |
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