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The Antigenicity of Ex Vivo Cultivated Human Corneal Limbal Epithelial and Stromal Cells: Temporal Changes In Vitro

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dc.contributor.authorOh, Joo Youn-
dc.contributor.authorKo, Jung Hwa-
dc.contributor.authorLee, Hyun Ju-
dc.contributor.authorKim, Mee Kum-
dc.contributor.authorWee, Won Ryang-
dc.contributor.authorLee, Jin Hak-
dc.date.accessioned2012-06-28T09:03:16Z-
dc.date.available2012-06-28T09:03:16Z-
dc.date.issued2010-11-
dc.identifier.citationCORNEA; Vol.29 11; 1302-1307ko_KR
dc.identifier.issn0277-3740-
dc.identifier.urihttp://hdl.handle.net/10371/77814-
dc.description.abstractPurpose: To investigate the effect of ex vivo culture on the expression of human leukocyte antigen (HLA) in human corneal limbal epithelial cells (LECs), and to evaluate the expression of HLA and costimulatory molecules on human corneal limbal stromal cells (LSCs). Methods: The expression of HLA-ABC and HLA-DR were evaluated using flow cytometry on cultured human LECs and LSCs after serial passage and compared with that on freshly isolated cells. Additionally, the expression of costimulatory molecules CD80, CD86, CD40, and immunoregulatory molecule HLA-G were investigated on LSCs with or without an addition of interferon (IFN) gamma (250 U/mL, 4 days). Results: HLA-ABC and HLA-DR were expressed in 99.12 +/- 1.02% and 25.86 +/- 4.34% of freshly isolated LECs, respectively. After culture, the HLA-DR-expressing cells significantly decreased with serial passage, whereas the percentage of HLA-ABC-expressing cells did not change. More than 90% of LSCs expressed HLA-ABC and HLA-G, whereas the expression of HLA-DR, CD80, CD86, and CD40 was negligible. This expression profile did not change after serial passage. However, the treatment with IFN-gamma induced a significant increase in HLA-DR (0.17% vs. 17.68%) and CD40 (0.19% vs. 13.19%) expression on LSCs. Conclusions: The immunogenicity of LECs can be lowered after ex vivo cultivation, whereas LSCs can be immunogenic in the presence of IFN-gamma.ko_KR
dc.language.isoenko_KR
dc.publisherLIPPINCOTT WILLIAMS & WILKINSko_KR
dc.subjectcorneal stromal cellko_KR
dc.subjectlimbal epithelial cellko_KR
dc.subjecthuman leukocyte antigenko_KR
dc.subjectcostimulatory moleculeko_KR
dc.subjectcultivationko_KR
dc.titleThe Antigenicity of Ex Vivo Cultivated Human Corneal Limbal Epithelial and Stromal Cells: Temporal Changes In Vitroko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor오주윤-
dc.contributor.AlternativeAuthor고정화-
dc.contributor.AlternativeAuthor이현주-
dc.contributor.AlternativeAuthor김미금-
dc.contributor.AlternativeAuthor이진학-
dc.contributor.AlternativeAuthor위원량-
dc.identifier.doi10.1097/ICO.0b013e3181e3f01e-
dc.citation.journaltitleCORNEA-
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College of Medicine/School of Medicine (의과대학/대학원)Ophthalmology (안과학전공)Journal Papers (저널논문_안과학전공)
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