인간 면역결핍 바이러스 양성환자의 항문부 첨규 콘딜로마에서 관찰되는 인유두종 바이러스의 아형에 관한 연구

Abstract

Purpose: Anal condyloma is an epithelial proliferative lesion caused by human papillomavirus (HPV) infection. The present study analyzed the HPV types detected in HIV (+) Korean anal condyloma using PCR-based DNA microarray. Methods: DNA was extracted from the condyloma tissue of 1.7 patients including 9 HIV (+) patients (M : F=15 : 2, mean age 35 years, 22 similar to 59 years). The 1st PCR was performed with a general primer on L1 region, and nested PCR on the products of the 1st PCR. PCR products were hybridized with a DNA chip. Results: Fourteen patients (9 HIV (+), 5 HIV (-)) showed positive HPV DNA. Overall, type 6 was the most common (N=11), and type 11. (N=6), type 53 (N=3) in order. Among HIV (+) patients, type 6 was also the most common (N=7), then type 11 (N=5) and type 53 (N=3). In contrast to the HIV (-) patients, 5 patients (55.6%) proved to have multiple infections in HIV (+) patients (2 double, 2 triple, 1 quadruple infection). Four of 9 HIV (+) patients (44.5%) showed co-infection with high-risk HPV. Conclusion: Multiple infection and co-infection with high-risk types are more prevalent in HIV (+) condyloma patients compared to HIV (-) patients. HPV types on HIV (+) male anal condyloma arc similar to those detected in the Korean female uterine cervix. (J Korean Surg Soc 2010;78:111-115)