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Quantum-dots-based detection of hepatitis C virus (HCV) NS3 using RNA aptamer on chip
DC Field | Value | Language |
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dc.contributor.author | Roh, Changhyun | - |
dc.contributor.author | Lee, Ho-Young | - |
dc.contributor.author | Kim, Sang-Eun | - |
dc.contributor.author | Jo, Sung-Kee | - |
dc.date.accessioned | 2012-07-03T08:47:30Z | - |
dc.date.available | 2012-07-03T08:47:30Z | - |
dc.date.issued | 2010-08 | - |
dc.identifier.citation | JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY; Vol.85 8; 1130-1134 | ko_KR |
dc.identifier.issn | 0268-2575 | - |
dc.identifier.uri | https://hdl.handle.net/10371/78324 | - |
dc.description.abstract | BACKGROUND: Over 170 million people, more than 3% of the world`s population, suffer from the hepatitis C virus (HCV) infection and the rate of death from liver-related mortality to HCV has increased. In respect of this, the development of assays for biological imaging should be urgently considered as an essential factor in diagnosis. RESULTS: A novel HCV-detecting technique using a nanoparticle-supported aptamer probe was demonstrated. With the aid of nanoparticle quantum dots (QDs) with carboxyl group as an imaging probe, and 5`-end-amine-modified RNA oligonucleotide as a capturing probe, target HCV NS3 was visually detected on chip. The QDs-based RNA aptamer for HCV NS3 showed high selectivity and specificity against other protein such as BSA. The detection limit of HCV N53 protein was 5 ng mL(-1) level. CONCLUSION: With a novel strategy for protein-aptamer interaction, the feasibility of applying QDs-based fluorescent detection technique to HCV viral protein assay for the development of a protein biochip was demonstrated. This scheme of QDs-mediated imaging with a target-oriented specific RNA aptamer for the detection of infectious HCV diseases provides an efficient strategy and a promising new platform for monitoring applications. (C) 2010 Society of Chemical Industry | ko_KR |
dc.description.sponsorship | This work
was supported by Nuclear R&D Program of Ministry of Education, Science and Technology, South Korea. It was also supported by SNU-KAERI Degree and Research Center for Radiation Convergence Sciences from Korea Research Council of Foundation (KRCF) Science & Technology, South Korea. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | JOHN WILEY & SONS LTD | ko_KR |
dc.subject | nanoparticle | ko_KR |
dc.subject | quantum dots (QDs) | ko_KR |
dc.subject | imaging | ko_KR |
dc.subject | diagnosis | ko_KR |
dc.subject | RNA aptamer | ko_KR |
dc.subject | hepatitis C virus (HCV) | ko_KR |
dc.title | Quantum-dots-based detection of hepatitis C virus (HCV) NS3 using RNA aptamer on chip | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 노창현 | - |
dc.contributor.AlternativeAuthor | 이호영 | - |
dc.contributor.AlternativeAuthor | 김상은 | - |
dc.contributor.AlternativeAuthor | 조성기 | - |
dc.identifier.doi | 10.1002/jctb.2409 | - |
dc.citation.journaltitle | JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY | - |
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dc.description.tc | 2 | - |
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