Implantation of canine umbilical cord blood-derived mesenchymal stem cells mixed with beta-tricalcium phosphate enhances osteogenesis in bone defect model dogs

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Jang, Byung-Jun; Byeon, Ye-Eun; Lim, Ji-Hey; Ryu, Hak-Hyun; Kim, Wan Hee; Koyama, Yoshihisa; Kikuchi, Masanori; Kang, Kyung-Sun; Kweon, Oh Kyeong
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대한수의학회 = The Korean Society of Veterinary Science
J Vet Sci 2008, 9, 387-393
β-TCPdogmesenchymal stem cellosteogenesisumbilical cord blood
This study was performed to evaluate the osteogenic effect of
allogenic canine umbilical cord blood-derived mesenchymal
stem cells (UCB-MSCs) mixed with beta-tricalcium phosphate
(β-TCP) in orthotopic implantation. Seven hundred milligrams
of β-TCP mixed with 1 × 106 UCB-MSCs diluted with
0.5 ml of saline (group CM) and mixed with the same volume
of saline as control (group C) were implanted into a 1.5 cm
diaphyseal defect and wrapped with PLGC membrane in the
radius of Beagle dogs. Radiographs of the antebrachium
were made after surgery. The implants were harvested 12
weeks after implantation and specimens were stained with
H&E, toluidine blue and Villanueva-Goldner stains for
histological examination and histomorphometric analysis of
new bone formation. Additionally, UCB-MSCs were applied
to a dog with non-union fracture. Radiographically, continuity
between implant and host bone was evident at only one of six
interfaces in group C by 12 weeks, but in three of six interfaces
in group CM. Radiolucency was found only near the bone
end in group C at 12 weeks after implantation, but in the
entire graft in group CM. Histologically, bone formation
was observed around β-TCP in longitudinal sections of
implant in both groups. Histomorphometric analysis revealed
significantly increased new bone formation in group CM at
12 weeks after implantation (p < 0.05). When applied to the
non-union fracture, fracture healing was identified by 6
weeks after injection of UCB-MSCs. The present study
indicates that a mixture of UCB-MSCs and β-TCP is a
promising osteogenic material for repairing bone defects.
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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