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Electrical Stimulation-Induced Cell Clustering in Cultured Neural Networks

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Authors
Jun, Sang Beom; Hynd, Matthew R.; Smith, Karen L.; Song, Jong Keun; Turner, James N.; Shain, William; Kim, Sung June
Issue Date
2007-11
Publisher
Springer Verlag
Citation
Med Biol Eng Comput 45:1015-1021
Keywords
Neural networkMicroelectrode arrayElectrical stimulation
Abstract
Planar microelectrode arrays (MEAs) are
widely used to record electrical activity from neural networks.
However, only a small number of functional
recording sites frequently show electrical activity. One
contributing factor may be that neurons in vitro receive
insufficient synaptic input to develop into fully functional
networks. In this study, electrical stimulation was applied
to neurons mimicking synaptic input. Various stimulation
paradigms were examined. Stimulation amplitude and
frequency were tailored to prevent cell death. Two effects
of stimulation were observed when 3-week-old cultures
were stimulated: (1) clusters of neural cells were observed
adjacent to stimulating electrodes and (2) an increase in
spontaneous neuronal activity was recorded at stimulating
electrodes. Immunocytochemical analysis indicates that
stimulation may cause both new neuron process growth as
well as astrocyte activation. These data indicate that electrical
stimulation can be used as a tool to modify neural
networks at specific electrode sites and promote electrical
activity.
ISSN
0140-0118 (print)
1741-0444 (online)
Language
English
URI
http://hdl.handle.net/10371/7925
DOI
https://doi.org/10.1007/s11517-007-0218-6
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Appears in Collections:
College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Electrical and Computer Engineering (전기·정보공학부)Journal Papers (저널논문_전기·정보공학부)
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