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Effective neutrophil chemotaxis is strongly influenced by mean IL-8 concentration

Cited 108 time in Web of Science Cited 112 time in Scopus
Authors
Lin, Francis; Nguyen, Connie Minh-Canh; Wang, Shur-Jen; Saadi, Wajeeh; Gross, Steven P.; Jeon, Noo Li
Issue Date
2004-05-21
Publisher
Elsevier
Citation
Biochem. Biophys. Res. Commun. 319 (2004) 576-581
Keywords
chemotaxisgradientIL-8microfluidicsNeutrophil
Abstract
Neutrophils need to correctly interpret gradients of chemotactic factors (CFs) such as interleukin 8 (IL-8) to migrate to the site of
infection and perform immune functions. Because diffusion-based chemotaxis assays used in previous studies suffer from temporally
changing gradients, it is difficult to distinguish the influence of CF gradient steepness from mean CF concentration on chemotaxis.
To better understand the roles of mean CF concentration and CF gradient steepness, we developed a microfluidic device that can
maintain stable IL-8 gradients. We report that the random motility of neutrophils is a biphasic function of IL-8 concentration and
its magnitude plays a decisive role in effective chemotaxis, a quantitative measure of migration. We show that the concentrations for
the optimum chemotaxis in linear IL-8 gradients and for the maximum random motility in uniform IL-8 coincide. In contrast, we
find that the steepness of IL-8 gradients has no significant effect on effective chemotaxis.
2004 Elsevier Inc. All rights reserved.
ISSN
0006-291X
Language
English
URI
http://hdl.handle.net/10371/7978
DOI
https://doi.org/10.1016/j.bbrc.2004.05.029
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Mechanical Aerospace Engineering (기계항공공학부)Journal Papers (저널논문_기계항공공학부)
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