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Patterned cell culture inside microfluidic devices

Cited 222 time in Web of Science Cited 231 time in Scopus
Authors
Rhee, Seog Woo; Taylor, Anne M.; Tu, Christina H.; Cribbs, David H.; Cotman, Carl W.; Jeon, Noo Li
Issue Date
2004-07-26
Publisher
Royal Society of Chemistry
Citation
Lab Chip, 2004, 5, 102-107
Keywords
patternmicrofluidic device
Abstract
This paper describes a simple plasma-based dry etching method that enables patterned cell culture
inside microfluidic devices by allowing patterning, fluidic bonding and sterilization steps to be
carried out in a single step. This plasma-based dry etching method was used to pattern celladhesive
and non-adhesive areas on the glass and polystyrene substrates. The patterned substrate
was used for selective attachment and growth of human umbilical vein endothelial cells, MDAMB-
231 human breast cancer cells, NIH 3T3 mouse fibroblasts, and primary rat cortical neurons.
Finally, we have successfully combined the dry-patterned substrate with a microfluidic device.
Patterned primary rat neurons were maintained for up to 6 days inside the microfluidic devices
and the neurons’ somas and processes were confined to the cell-adhesive region. The method
developed in this work offers a convenient way of micropatterning biomaterials for selective
attachment of cells on the substrates, and enables culturing of patterned cells inside microfluidic
devices for a number of biological research applications where cells need to be exposed to wellcontrolled
fluidic microenvironment.
ISSN
1473-0197
Language
English
URI
http://hdl.handle.net/10371/7982
DOI
https://doi.org/10.1039/b403091e
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Mechanical Aerospace Engineering (기계항공공학부)Journal Papers (저널논문_기계항공공학부)
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