약물반응의 진단에 대한 연층백혈구 응집검사법의 응용에 관한 연구

Abstract

Buffy coat leucocyte aggregation (BCLA) in the presence of a specific antigen was suggested a simple. sensitive and highly reproducible in vitro method to test the cell-mediated immunity to certain antigens. The cell-mediated immune response is known to be responsible for certain exanthematous drug reaction and contact hypersensitivity to chemicals. The present study was undertaken to evaluate the BCLA test as a possible in vitro diagnostic aid to detect the drug reactions and contact hypersensitivity to certain chemicals. Twenty-two patients with ampicillin drug eruption, contact dermatitis to nickel sulfate, paraphenylenediamine, potassium dichromate and mercuric chloride and 68 control subjects entered this study. The method of the BCLA test was same as described by the authors elsewhere (]979). At various concentrations of cah antigen ranging from 2. 5 X 10 to 2.5 x 10-' mg/ml, none of the patients and the control subjects showed the BCLA. The authors concluded that in these antigen systems the BCLA test could not be a diagnostic method. The possible mechanism of this negative results was discussed.