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Human Periodontal Ligament Cell Response to a Newly Developed Calcium Phosphate-based Root Canal Sealer

Cited 26 time in Web of Science Cited 30 time in Scopus
Authors
Bae, Won-Jung; Chang, Seok-Woo; Lee, Sang-Im; Kum, Kee-Yeon; Kim, Eun-Cheol; Bae, Kwang-Shik
Issue Date
2010-10
Publisher
ELSEVIER SCIENCE INC
Citation
JOURNAL OF ENDODONTICS, Vol.36, No.10, pp.1658-1663
Keywords
Attachmentsinflammatory mediatorodontoblastic differentiationhuman dental pulp cellsCAPSEALcytotoxicity
Abstract
Objectives: The aim of this study was to investigate the cellular effects of newly developed calcium phosphate based sealers (CAPSEAL I and II) using cultured human periodontal ligament cells (HPDLCs) compared with epoxy resin sealer (AH26; Dentsply, DeTrey, Konstanz, Germany), zinc oxide eugenol [ZOE] sealer (extended working time [EWT]; Kerr Corporation, Orange, CA), and CPC sealer (Sankin apatite sealer; Sankin-kogyo, Tokyo, Japan). Methods: Cell viability by -(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazoliumbromide assay, cell attachment by scanning electron microscopy, osteoblastic differentiation and inflammatory mediators by reverse-transcriptase polymerase chain reaction, Western blotting, and alizarin red staining were evaluated. Results: The cytotoxicities of CAPSEAL I and II were less than those of AH 26 and EWT after 1 and 14 days. Cells on CAPSEAL 1 and II were spread better as compared with those on other sealers. Mineralization after 14 days and the expression of osteoblastic differentiation markers such as alkaline phosphate and osteonectin messenger RNA increased in CAPSEAL I- and II-exposed HPDLCs after 1 and 3 days, whereas the production of inflammatory mediators, including cyclooxygeanse-2, inducible nitric oxide synthetase, and reactive oxygen species (ROS), were lower than in other sealers. Conclusions: These results suggest that both CAPSEAL I and II show less cytotoxicity and inflammatory mediators compared with other sealers and have the potential to promote bone regeneration as root canal sealers. (J Endod 2010;36:1658-1663)
ISSN
0099-2399
Language
English
URI
http://hdl.handle.net/10371/80368
DOI
https://doi.org/10.1016/j.joen.2010.06.022
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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