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Effects of pilocarpine on the secretory acinar cells in human submandibular glands
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Li, Jinchao | - |
dc.contributor.author | Lee, Soojung | - |
dc.contributor.author | Choi, Se-Young | - |
dc.contributor.author | Lee, Jong-Ho | - |
dc.contributor.author | Chung, Sung Chang | - |
dc.contributor.author | Oh, Seog-Bae | - |
dc.contributor.author | Lee, Sung Joong | - |
dc.contributor.author | Kim, Joong-Soo | - |
dc.contributor.author | Park, Kyungpyo | - |
dc.contributor.author | Lee, Jong Heun | - |
dc.date.accessioned | 2013-01-14T07:10:26Z | - |
dc.date.available | 2013-01-14T07:10:26Z | - |
dc.date.issued | 2006-11-25 | - |
dc.identifier.citation | LIFE SCIENCES, Vol.79, No.26, pp.2441-2447 | ko_KR |
dc.identifier.issn | 0024-3205 | - |
dc.identifier.uri | https://hdl.handle.net/10371/80449 | - |
dc.description.abstract | Pilocarpine has been used as a choice of drugs for treatment of impaired salivary flow. Although considerable data are available as to the stimulatory effect of pilocarpine on the salivary secretion in human, its underlying mechanism, at the cellular level, has not been rigorously studied. In this experiment, we studied the effect of pilocarpine on the ion channel activity, cytoplasmic free Ca2+ concentration([Ca2+](i)) and aquaporin (AQP)-5 expression, which play key roles in the secretary process and determine the capacity of fluid secretion. In human submandibular gland (SMG) acinar cells, 10(-5) M pilocarpine activated the outward rectifying-current, which was predominantly K+ selective in the whole cell patch clamp study. The pilocarpine increased [Ca2+](i) in a concentration-dependent manner in the range of 10(-6) M to 10(-4) M. We found that both increases of [Ca2+](i) and outward rectifying-K+ current were inhibited by 10(-5) M U-73122, a specific phospholipase C inhibitor. The magnitudes of pilocarpine-induced [Ca2+](i) transients were approximately, 5 5 % lower than those with the same concentration of carbachol (CCh). Pilocarpine also increased the amount of AQP-5 protein in the apical membrane (APM) in human SMG acinar cells. Our results suggest that pilocarpine induce salivary secretions in human by activating K+ channels, increasing [Ca2+](i) via phospholipase C dependent pathway, and increasing AQP-5 protein expression in the APM, of SMG acinar cells. (c) 2006 Elsevier Inc. All rights reserved. | ko_KR |
dc.description.sponsorship | This work was supported by a grant of the Korea Health 21 R and D project, Ministry of Health and Welfare, Republic of Korea (Grant No 01-PJ5-PG1-01CH12-0002). | - |
dc.language.iso | en | ko_KR |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | ko_KR |
dc.subject | pilocarpine | ko_KR |
dc.subject | ion channel | ko_KR |
dc.subject | cytoplasmic free Ca2+ concentration ([Ca2+](i)) | ko_KR |
dc.subject | aquaporin-5 | ko_KR |
dc.subject | submandibular gland(SMG) | ko_KR |
dc.title | Effects of pilocarpine on the secretory acinar cells in human submandibular glands | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 이수정 | - |
dc.contributor.AlternativeAuthor | 최세영 | - |
dc.contributor.AlternativeAuthor | 이종호 | - |
dc.contributor.AlternativeAuthor | 정성창 | - |
dc.contributor.AlternativeAuthor | 오석배 | - |
dc.contributor.AlternativeAuthor | 이성중 | - |
dc.contributor.AlternativeAuthor | 김중수 | - |
dc.contributor.AlternativeAuthor | 박경표 | - |
dc.contributor.AlternativeAuthor | 이종흔 | - |
dc.identifier.doi | 10.1016/j.lfs.2006.08.006 | - |
dc.citation.journaltitle | LIFE SCIENCES | - |
dc.description.tc | 3 | - |
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