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Crosstalk between Nuclear Factor I-C and Transforming Growth Factor-b1 Signaling Regulates Odontoblast Differentiation and Homeostasis
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Dong-Seol | - |
dc.contributor.author | Yoon, Won-Joon | - |
dc.contributor.author | Cho, Eui Sic | - |
dc.contributor.author | Kim, Heung-Joong | - |
dc.contributor.author | Gronostajski, Richard M. | - |
dc.contributor.author | Cho, Moon-Il | - |
dc.contributor.author | Park, Joo-Cheol | - |
dc.creator | 박주철 | - |
dc.date.accessioned | 2013-04-11T01:56:23Z | - |
dc.date.available | 2013-04-11T01:56:23Z | - |
dc.date.issued | 2011-12 | - |
dc.identifier.citation | PLOS ONE Vol.6 No.12, e29160 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://hdl.handle.net/10371/81913 | - |
dc.description.abstract | Transforming growth factor-b1 (TGF-b1) signaling plays a key role in vertebrate development, homeostasis, and disease.Nuclear factor I-C (NFI-C) has been implicated in TGF-b1 signaling, extracellular matrix gene transcription, and tooth rootdevelopment. However, the functional relationship between NFI-C and TGF-b1 signaling remains uncharacterized. Thepurpose of this study was to identify the molecular interactions between NFI-C and TGF-b1 signaling in mouseodontoblasts. Real-time polymerase chain reaction and western analysis demonstrated that NFI-C expression levels wereinversely proportional to levels of TGF-b1 signaling molecules during in vitro odontoblast differentiation. Western blot andimmunofluorescence results showed that NFI-C was significantly degraded after TGF-b1 addition in odontoblasts, and theformation of the Smad3 complex was essential for NFI-C degradation. Additionally, ubiquitination assay results showed thatSmurf1 and Smurf2 induced NFI-C degradation and polyubiquitination in a TGF-b1-dependent manner. Both kinase and invitro binding assays revealed that the interaction between NFI-C and Smurf1/Smurf2 requires the activation of the mitogenactivatedprotein kinase pathway by TGF-b1. Moreover, degradation of NFI-C induced by TGF-b1 occurred generally in celltypes other than odontoblasts in normal human breast epithelial cells. In contrast, NFI-C induced dephosphorylation of p-Smad2/3. These results show that crosstalk between NFI-C and TGF-b1 signaling regulates cell differentiation andhomeostatic processes in odontoblasts, which might constitute a common cellular mechanism. | en |
dc.language.iso | en | en |
dc.publisher | PUBLIC LIBRARY OF SCIENCE | en |
dc.subject | 자연과학 | en |
dc.title | Crosstalk between Nuclear Factor I-C and Transforming Growth Factor-b1 Signaling Regulates Odontoblast Differentiation and Homeostasis | en |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 이동설 | - |
dc.contributor.AlternativeAuthor | 윤원준 | - |
dc.contributor.AlternativeAuthor | 조의식 | - |
dc.contributor.AlternativeAuthor | 김흥중 | - |
dc.contributor.AlternativeAuthor | 조문일 | - |
dc.contributor.AlternativeAuthor | 박주철 | - |
dc.identifier.doi | 10.1371/journal.pone.0029160 | - |
dc.description.srnd | OAIID:oai:osos.snu.ac.kr:snu2011-01/102/0000042428/7 | - |
dc.description.srnd | SEQ:7 | - |
dc.description.srnd | PERF_CD:SNU2011-01 | - |
dc.description.srnd | EVAL_ITEM_CD:102 | - |
dc.description.srnd | USER_ID:0000042428 | - |
dc.description.srnd | ADJUST_YN:N | - |
dc.description.srnd | EMP_ID:A077448 | - |
dc.description.srnd | DEPT_CD:861 | - |
dc.description.srnd | CITE_RATE:4.092 | - |
dc.description.srnd | FILENAME:PLOS one-crosstalk - CR.pdf | - |
dc.description.srnd | DEPT_NM:치의학과 | - |
dc.description.srnd | EMAIL:jcapark@snu.ac.kr | - |
dc.description.srnd | SCOPUS_YN:N | - |
dc.description.srnd | CONFIRM:Y | - |
dc.identifier.srnd | 2011-01/102/0000042428/7 | - |
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