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TGF-beta 1 (transforming growth factor-beta 1)-mediated adhesion of gastric carcinoma cells involves a decrease in Ras/ERKs (extracellular-signal-regulated kinases) cascade activity dependent on c-Src activity

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dc.contributor.authorKim, Hwang-Phill-
dc.contributor.authorLee, Mi-Sook-
dc.contributor.authorYu, Jiyon-
dc.contributor.authorPark, Jin-Ah-
dc.contributor.authorJong, Hyun-Soon-
dc.contributor.authorKim, Tae-You-
dc.contributor.authorLee, Jung Weon-
dc.contributor.authorBang, Yung-Jue-
dc.creator이정원-
dc.date.accessioned2013-04-16T07:51:45Z-
dc.date.available2013-04-16T07:51:45Z-
dc.date.issued2004-04-
dc.identifier.citationBIOCHEMICAL JOURNAL Vol.379 No.1, pp. 141-150-
dc.identifier.issn0264-6021-
dc.identifier.urihttp://hdl.handle.net/10371/82043-
dc.description.abstractSignalling by integrin-mediated cell anchorage to extracellular matrix proteins is co-operative with other receptor-mediated signalling pathways to regulate cell adhesion, spreading, proliferation survival. migration. differentiation and gene expression. It was observed that an anchorage-independent gastric carcinoma cell line (SNU16) became adherent on TGF-beta1 (transforming growth factor beta1)treatment. To undertstand how a signal cross-talk between integrin and TGF-beta1 path ways forms the basis for TGF-beta1 effects, cell adhesion and signalling activities were studied using an adherent subline (SNU16Ad. an adherent variant cell line derived from SNU16) derived from the SNU16 cells. SNU16 and SNU16Ad cells, but not integrin alpha5-expressing SNU16 cells, showed an increase in adhesion on extracellular matrix proteins after TGF-beta1 treatment. This increase was shown to be mediated by an integrin alpha3 subunit. Which Was up-regulated in adherent SNU I 6Ad cells and in TGF-beta1-treated SNU16 cells, compared with the parental SNU16 cells. After TGF-beta1 treatment of SNU I 6Ad cells on fibronectin. Tyr-416 phosphorylation of c-Src was increased, but Ras-GTP loading and ERK1/ERK2 (extra-cellular-signal-regulated kinases 1 and 2) activity were decreased, which showed a dependence on c-Src family kinase activity. Studies on adhesion and signalling activities using pharmacological inhibitors or by transient-transfection approaches showed that inhibition of ERK1/ERK2 activity increased TGF-beta1 mediated cell adhesion slightly, but not the basal cell adhesion significantly, and that c-Src family kinase activity and decrease in Ras/ERKs cascade activity were required for the TGF-beta1 effects. Altogether, the present Study indicates that TGF-beta1 treatment causes anchorage-independent gastric carcinoma cells to adhere by an increase in integrin alpha3 level and a c-Src family kinase activity-dependent decrease in Ras/ERKs cascade activity.en
dc.language.isoenen
dc.publisherPortland Pressen
dc.subject복합학en
dc.subjectcell adhesionen
dc.subjectc-Src-
dc.subjectextracellular-signal-regulated
kinases 1 and 2 (ERK1/ERK2),
-
dc.subjectgastric carcinoma-
dc.subjecttransforming
growth factor β1 (TGF-β1)
-
dc.subject.otherintegrin-
dc.titleTGF-beta 1 (transforming growth factor-beta 1)-mediated adhesion of gastric carcinoma cells involves a decrease in Ras/ERKs (extracellular-signal-regulated kinases) cascade activity dependent on c-Src activityen
dc.typeArticle-
dc.contributor.AlternativeAuthor김황필-
dc.contributor.AlternativeAuthor이미숙-
dc.contributor.AlternativeAuthor유지연-
dc.contributor.AlternativeAuthor박진아-
dc.contributor.AlternativeAuthor종현순-
dc.contributor.AlternativeAuthor김태유-
dc.contributor.AlternativeAuthor이정원-
dc.contributor.AlternativeAuthor방영주-
dc.identifier.doi10.1042/BJ20031408-
dc.description.srndOAIID:oai:osos.snu.ac.kr:snu2004-01/102/0000003910/2-
dc.description.srndSEQ:2-
dc.description.srndPERF_CD:SNU2004-01-
dc.description.srndEVAL_ITEM_CD:102-
dc.description.srndUSER_ID:0000003910-
dc.description.srndADJUST_YN:Y-
dc.description.srndEMP_ID:A078142-
dc.description.srndDEPT_CD:375-
dc.description.srndCITE_RATE:4.278-
dc.description.srndFILENAME:15HPK_BJ2004.pdf-
dc.description.srndDEPT_NM:약학과-
dc.description.srndEMAIL:jwl@snu.ac.kr-
dc.description.srndCONFIRM:Y-
dc.identifier.rimsid4233-
dc.identifier.srnd2004-01/102/0000003910/2-
dc.type.rimsART-
Appears in Collections:
College of Pharmacy (약학대학)Dept. of Pharmacy (약학과)Journal Papers (저널논문_약학과)
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