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Cross-talk between TGFβ1 and EGFR signaling pathways induces TM4SF5 expression and Epithelial-Mesenchymal Transition.
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kang, Minkyung | - |
dc.contributor.author | Choi, Suyong | - |
dc.contributor.author | Jeong, Soo-Jin | - |
dc.contributor.author | Lee, Sin-Ae | - |
dc.contributor.author | Kwak, Tae Kyoung | - |
dc.contributor.author | Kim, Hyeonjung | - |
dc.contributor.author | Jung, Oisun | - |
dc.contributor.author | Lee, Mi-Sook | - |
dc.contributor.author | Park SY | - |
dc.contributor.author | Ko, Youra | - |
dc.contributor.author | Ryu, Jihye | - |
dc.contributor.author | Jeong, Doyoung | - |
dc.contributor.author | Lee, Hyo Jeong | - |
dc.contributor.author | Ye, Sang-Kyu | - |
dc.contributor.author | Kim, Sung-Hoon | - |
dc.contributor.author | Lee, Jung Weon | - |
dc.contributor.author | Choi, Yoon-Ju | - |
dc.creator | 이정원 | - |
dc.date.accessioned | 2013-04-16T07:54:15Z | - |
dc.date.available | 2013-04-16T07:54:15Z | - |
dc.date.issued | 2012-05 | - |
dc.identifier.citation | BIOCHEMICAL JOURNAL Vol.443 No.3, pp. 691-700 | - |
dc.identifier.issn | 0264-6021 | - |
dc.identifier.uri | https://hdl.handle.net/10371/82068 | - |
dc.description.abstract | The EMT (epithelial–mesenchymal transition) is involved in
fibrosis and cancer, and is regulated by different signalling pathways mediated through soluble factors, actin reorganization and transcription factor actions. Because the tetraspan (also called tetraspanin) TM4SF5 (transmembrane 4 L6 family member 5) is highly expressed in hepatocellular carcinoma and induces EMT, understanding how TM4SF5 expression in hepatocytes is regulated is important. We explored the mechanisms that induce TM4SF5 expression and whether impaired signalling pathways for TM4SF5 expression inhibit the acquisition of mesenchymal cell features, using human and mouse normal hepatocytes. We found that TGFβ1 (transforming growth factor β1)-mediated Smad activation caused TM4SF5 expression and EMT, and activation of the EGFR [EGF (epidermal growth factor) receptor] pathway. Inhibition of EGFR activity following TGFβ1 treatment abolished acquisition of EMT, suggesting a link from Smads to EGFR for TM4SF5 expression. Further, TGFβ1-mediated EGFR activation and TM4SF5 expression were abolished by EGFR suppression or extracellular EGF depletion. Smad overexpression mediated EGFR activation and TM4SF5 expression in the absence of serum, and EGFR kinase inactivation or EGF depletion abolished Smad-overexpression-induced TM4SF5 and mesenchymal cell marker expression. Inhibition of Smad, EGFR or TM4SF5 using Smad7 or small compounds also blocked TM4SF5 expression and/or EMT. These results indicate that TGFβ1- and growth factor-mediated signalling activities mediate TM4SF5 expression leading to acquisition of mesenchymal cell features, suggesting that TM4SF5 induction may be involved in the development of liver pathologies. | en |
dc.language.iso | en | en |
dc.publisher | Portland Press | en |
dc.subject | 복합학 | en |
dc.subject | epidermal growth factor receptor (EGFR) | - |
dc.subject | epithelial–
mesenchymal transition (EMT) | - |
dc.subject | gene induction | - |
dc.subject | signalling
cross-talk | - |
dc.subject | tetraspanin | - |
dc.subject | transforming growth factor β1 (TGFβ1) | - |
dc.title | Cross-talk between TGFβ1 and EGFR signaling pathways induces TM4SF5 expression and Epithelial-Mesenchymal Transition. | en |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 강민경 | - |
dc.contributor.AlternativeAuthor | 최수용 | - |
dc.contributor.AlternativeAuthor | 정수진 | - |
dc.contributor.AlternativeAuthor | 이신애 | - |
dc.contributor.AlternativeAuthor | 곽태경 | - |
dc.contributor.AlternativeAuthor | 김현정 | - |
dc.contributor.AlternativeAuthor | 정외선 | - |
dc.contributor.AlternativeAuthor | 이미숙 | - |
dc.contributor.AlternativeAuthor | 고유라 | - |
dc.contributor.AlternativeAuthor | 류지혜 | - |
dc.contributor.AlternativeAuthor | 최윤주 | - |
dc.contributor.AlternativeAuthor | 정도영 | - |
dc.contributor.AlternativeAuthor | 이효정 | - |
dc.contributor.AlternativeAuthor | 예상규 | - |
dc.contributor.AlternativeAuthor | 김성훈 | - |
dc.contributor.AlternativeAuthor | 이정원 | - |
dc.identifier.doi | 10.1042/BJ20111584 | - |
dc.description.srnd | OAIID:oai:osos.snu.ac.kr:snu2012-01/102/0000003910/3 | - |
dc.description.srnd | SEQ:3 | - |
dc.description.srnd | PERF_CD:SNU2012-01 | - |
dc.description.srnd | EVAL_ITEM_CD:102 | - |
dc.description.srnd | USER_ID:0000003910 | - |
dc.description.srnd | ADJUST_YN:N | - |
dc.description.srnd | EMP_ID:A078142 | - |
dc.description.srnd | DEPT_CD:375 | - |
dc.description.srnd | CITE_RATE:4.897 | - |
dc.description.srnd | FILENAME:66MKK-BJ.pdf | - |
dc.description.srnd | DEPT_NM:약학과 | - |
dc.description.srnd | EMAIL:jwl@snu.ac.kr | - |
dc.description.srnd | SCOPUS_YN:Y | - |
dc.description.srnd | CONFIRM:Y | - |
dc.identifier.srnd | 2012-01/102/0000003910/3 | - |
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