Publications
Detailed Information
Selective Aggregation Mechanism of Unmodified Gold Nanoparticles in Detection of Single Nucleotide Polymorphism
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho, Kyungnam | - |
dc.contributor.author | Lee, Yunhee | - |
dc.contributor.author | Lee, Chang-Ha | - |
dc.contributor.author | Lee, Kangtaek | - |
dc.contributor.author | Kim, Youngmin | - |
dc.contributor.author | Choi, Hyoungwoo | - |
dc.contributor.author | Ryu, Pan Dong | - |
dc.contributor.author | Lee, So Yeong | - |
dc.contributor.author | Joo, Sang-Woo | - |
dc.date.accessioned | 2009-08-31T23:14:11Z | - |
dc.date.available | 2009-08-31T23:14:11Z | - |
dc.date.issued | 2008-05-15 | - |
dc.identifier.citation | J. Phys. Chem. C. 2008, 112, 8629 | en |
dc.identifier.issn | 1932-7447 | - |
dc.identifier.uri | https://hdl.handle.net/10371/8272 | - |
dc.description.abstract | Detection of single point mutation based on the hybridization of oligonucleotides was performed using unmodified gold nanoparticles. The sequences of oligonucleotides were designed to detect the metastatic efficiency modifier signal-induced proliferation-associated gene 1 (Sipa1). The detection step was monitored using UV−vis absorption spectroscopy, quasielastic light scattering, and zeta potential measurement. We observed that addition of DNAs into the suspension of unmodified gold nanoparticles could substantially aggregate the gold nanoparticles and change the color of solution. By changing the salt concentration in the presence of a phosphate buffer solution, we were able to selectively aggregate gold nanoparticles for the perfectly matched DNA, which enabled a detection of perfectly matched DNA from the single point-mutated one. Our results indicate that a change in the electrostatic interaction is responsible for the selective aggregation of gold nanoparticles upon the addition of DNA. This suggests a novel design principle for a rapid detection of the DNA sequence by controlling the electrostatic interactions between gold nanoparticles. | en |
dc.description.sponsorship | K. L. thanks the financial support by the
Korea Science and Engineering Foundation (KOSEF) through grant R01-2007-000-20821-0 and National Core Research Center for Nanomedical Technology (R15-2004-024-00000-0). S.-W. J. thanks the financial support by the KOSEF (R01-2006- 000-10017-0), the Seoul R&BD program, and the Soongsil University Research Fund. The authors thank Soo Hwa Jang for help with polyacrylamide gel analysis. | en |
dc.language.iso | en | en |
dc.publisher | American Chemical Society | en |
dc.title | Selective Aggregation Mechanism of Unmodified Gold Nanoparticles in Detection of Single Nucleotide Polymorphism | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 조경남 | - |
dc.contributor.AlternativeAuthor | 이윤희 | - |
dc.contributor.AlternativeAuthor | 이창하 | - |
dc.contributor.AlternativeAuthor | 이강택 | - |
dc.contributor.AlternativeAuthor | 김영민 | - |
dc.contributor.AlternativeAuthor | 최형우 | - |
dc.contributor.AlternativeAuthor | 류판동 | - |
dc.contributor.AlternativeAuthor | 이소영 | - |
dc.contributor.AlternativeAuthor | 조상우 | - |
dc.identifier.doi | 10.1021/jp801078m | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.