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Detection of Antibodies Against SARS-Coronavirus Using Recombinant Truncated Nucleocapsid Proteins by ELISA
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Hyun Kyoung | - |
dc.contributor.author | Lee, Byoung Hee | - |
dc.contributor.author | Dutta, Noton Kumar | - |
dc.contributor.author | Seok, Seung Hyeok | - |
dc.contributor.author | Baek, Min Won | - |
dc.contributor.author | Lee, Hui Young | - |
dc.contributor.author | Kim, Dong Jae | - |
dc.contributor.author | Na, Yi Rang | - |
dc.contributor.author | Noh, Kyoung Jin | - |
dc.contributor.author | Park, Sung Hoon | - |
dc.contributor.author | Kariwa, Hiroaki | - |
dc.contributor.author | Nakauchi, Mina | - |
dc.contributor.author | Quynh Mai, Le | - |
dc.contributor.author | Heo, Suk-Jin | - |
dc.contributor.author | Park, Jae Hak | - |
dc.date.accessioned | 2009-08-31T23:58:25Z | - |
dc.date.available | 2009-08-31T23:58:25Z | - |
dc.date.issued | 2008 | - |
dc.identifier.citation | J. Microbiol. Biotechnol. 18:1717-1721 | en |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://hdl.handle.net/10371/8315 | - |
dc.description.abstract | Severe acute respiratory syndrome (SARS) is a lifethreatening emerging respiratory disease caused by the coronavirus, SARS-CoV. The nucleocapsid (N) protein of SARS-CoV is highly antigenic and may be a suitable candidate for diagnostic applications. We constructed truncated recombinant N proteins (N1 [1-422 aa], N2 [1- 109 aa], and N3 [110-422 aa]) and determined their antigenicity by Western blotting using convalescent SARS serum. The recombinants containing N1 and N3 reacted with convalescent SARS serum in Western blotting. However, the recombinant with N2 did not. In ELISA using N1 or N3 as the antigens, positive results were observed in 10 of 10 (100%) SARS-CoV-positive human sera. None of 50 healthy sera gave positive results in either assay. These data indicate that the ELISA using N1 or N3 has high sensitivity and specificity. These results suggest that the middle or C-terminal region of the SARS N protein is important for eliciting antibodies against SARS-CoV during the immune response, and ELISA reactions using N1 or N3 may be a valuable tool for SARS diagnosis. | en |
dc.description.sponsorship | This work was supported by grants-in-aid from the Korea food and drug administration and the Korea Research Foundation(KRF-005-E00077). In addition, this work was partially supported through the BK 21 Program for Veterinary Science. | en |
dc.language.iso | en | en |
dc.publisher | 한국미생물생명공학회 = The Korean Society for Applied Microbiology | en |
dc.subject | ELISA | en |
dc.subject | SARS | en |
dc.subject | truncated recombinant nucleocapsid proteins | en |
dc.title | Detection of Antibodies Against SARS-Coronavirus Using Recombinant Truncated Nucleocapsid Proteins by ELISA | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 이현경 | - |
dc.contributor.AlternativeAuthor | 이병희 | - |
dc.contributor.AlternativeAuthor | 석승혁 | - |
dc.contributor.AlternativeAuthor | 백민원 | - |
dc.contributor.AlternativeAuthor | 이희영 | - |
dc.contributor.AlternativeAuthor | 김동재 | - |
dc.contributor.AlternativeAuthor | 나이랑 | - |
dc.contributor.AlternativeAuthor | 노경진 | - |
dc.contributor.AlternativeAuthor | 박성훈 | - |
dc.contributor.AlternativeAuthor | 허석진 | - |
dc.contributor.AlternativeAuthor | 박재학 | - |
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