SHERP

Surface-Enhanced Raman Spectroscopic-Encoded Beads for Multiplex Immunoassay

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Authors
Jun, Bong-Hyun; Kim, Jong-Ho; Park, Hyunmi; Kim, Jun-Sung; Yu, Kyeong Nam; Lee, Sang-Myung; Choi, Heejeong; Kwak, Seon-Yeong; Kim, Yong-Kweon; Jeong, Dae Hong; Cho, Myung-Haing; Lee, Yoon-Sik
Issue Date
2007-02-14
Publisher
American Chemical Society
Citation
J. Comb. Chem. 2007, 9, 237-244
Abstract
A new type of encoded bead, which uses surface-enhanced Raman scattering (SERS), is described for
multiplex immunoassays. Silver nanoparticles were embedded in sulfonated polystyrene (PS) beads via a
polyol method, and they were used as SERS-active substrates. Raman-label organic compounds such as
4-methylbenzenethiol (4-MT), 2-naphthalenethiol (2-NT), and benzenethiol (BT) were then adsorbed onto
the silver nanoparticles in the sulfonated PS bead. Although only three kinds of encoding have been
demonstrated here, various combinations of these Raman-label organic compounds have the potential to
give a large number of tags. The Raman-label-incorporated particles were then coated with a silica shell
using tetraethoxyorthosilicate (TEOS) for chemical stability and biocompatibility. The resulting beads showed
unique and intense Raman signals for the labeled organic compounds. We demonstrated that SERS-encoded
beads could be used for multiplex detection with a model using streptavidin and p53. In our system, the
binding event of target molecules and the type of ligand can be simultaneously recognized by Raman
spectroscopy using a single laser-line excitation (514.5 nm).
ISSN
1520-4766
Language
English
URI
http://hdl.handle.net/10371/8336
DOI
https://doi.org/10.1021/cc0600831
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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